Purification of RNA from Cells and Tissues—NucleoSpin RNA Plus
The NucleoSpin RNA Plus kit is a next-generation RNA purification kit designed for quick, efficient RNA isolation from cells and tissues. This kit integrates a new feature, the NucleoSpin gDNA Removal Column, a mini spin column which quickly and efficiently removes genomic DNA, eliminating the need for time-consuming rDNase digestion.
Cells and tissues are lysed in a lysis buffer that does not require the addition of beta-mercapthoethanol or TCEP, since it contains a chaotropic salt that immediately inactivates any RNases. The lysate is added to a NucleoSpin gDNA Removal Column for clarification and removal of contaminating gDNA. After addition of the binding solution to the flowthrough, the RNA is bound to a NucleoSpin RNA Plus Column. Two washes are performed to remove salts, metabolites, and macromolecular cellular components, and high-quality RNA is eluted with RNase-free water.