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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  RNA >  Total_RNA_from_Cells_and_Tissues >  RNA_Plus_Miniprep

Purification of RNA from Cells and Tissues—NucleoSpin RNA Plus

The NucleoSpin RNA Plus kit is a next-generation RNA purification kit designed for quick, efficient RNA isolation from cells and tissues. This kit integrates a new feature, the NucleoSpin gDNA Removal Column, a mini spin column which quickly and efficiently removes genomic DNA, eliminating the need for time-consuming rDNase digestion.

Cells and tissues are lysed in a lysis buffer that does not require the addition of beta-mercapthoethanol or TCEP, since it contains a chaotropic salt that immediately inactivates any RNases. The lysate is added to a NucleoSpin gDNA Removal Column for clarification and removal of contaminating gDNA. After addition of the binding solution to the flowthrough, the RNA is bound to a NucleoSpin RNA Plus Column. Two washes are performed to remove salts, metabolites, and macromolecular cellular components, and high-quality RNA is eluted with RNase-free water.

  At-A-Glance   Documents   Images & Data

Features

  • Next-generation kit—provides quick, efficient RNA isolation from cells and tissues
  • NucleoSpin gDNA Column—eliminates the need for time-consuming rDNase digestion
  • Convenient handling—one-step lysate clearing and gDNA removal using a single column
  • Efficient lysis buffer—no beta-mercapthoethanol or TCEP needed

Applications

  • RNA isolation from cultured cells, animal tissues, plant tissues, microorganisms
  • Real-time RT-PCR, Northern blotting, primer extension, array technology, and RNase protection assays

Product Overview

Technology Two-column silica-membrane system
1. Column for DNA removal
2. Column for RNA isolation
Format Mini spin columns
Starting Material <1 x 107 cultured cells,
<109 bacterial cells,
 <108 yeast cells,
 <30 mg human/animal tissue
Fragment Size >200 nt
Typical Yield 5 x 106 HeLa cells: 40–60 µg
20 mg mouse liver: 80–100 µg
20 mg mouse kidney: 40–70 µg
5 mg mouse spleen: 30–60 µg
A260/A280 1.9–2.1
A260/A230 1.8–2.5
Typical RIN (RNA Integrity Number) >9
Elution Volume 30–120 µl
Preparation Time 20 min/6 preps
Binding Capacity 200 µg

 
 
Products
Cat. # Product Contents Size Price Units Select
740984.250 NucleoSpin® RNA Plus 250 Preps $1,216.00
740984.50 NucleoSpin® RNA Plus 50 Preps $275.00
 

 

 
 

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