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Products >  Nucleic_Acid_Purification >  RNA >  Total_RNA_from_Cells_and_Tissues >  NucleoZOL

RNA Isolation Reagent for miRNA and total RNA—NucleoZOL

NucleoZOL is a highly efficient RNA isolation reagent for extracting miRNA and total RNA from a variety of sample types, such as cultured cells, animal and plant tissue, yeast, bacteria, and viruses. A simple, one-phase extraction with NucleoZOL eliminates the need for an inconvenient two-phase separation using chloroform, which minimizes the risk of sample loss and phenol carryover. DNA, protein, and other contaminants are removed by precipitation. NucleoZOL can be used to isolate total RNA in a single fraction, or miRNA and large RNA in two separate fractions. The NucleoZOL procedure can also be used in combination with NucleoSpin RNA Columns.

The NucleoSpin RNA Set for NucleoZOL is a mini spin kit designed to combine NucleoZOL RNA extraction with NucleoSpin RNA purification. The kit contains silica membrane-based columns and the required buffers. After a sample is lysed in NucleoZOL (not provided in the mini spin kit), and contaminating DNA and proteins are pelleted, the supernatant is mixed with an optimized binding buffer for efficient binding of miRNA and large RNA to the silica membrane. A convenient bind-wash-elute procedure allows highly efficient isolation of pure RNA (miRNA and large RNA).

  At-A-Glance   Documents   Images & Data

Features

  • Simple procedure with no need for a phase separation using chloroform
  • High yields of highly pure RNA from a wide variety of sample types
  • Isolation of miRNA and large RNA in a single fraction or two separate fractions
  • One-phase RNA extraction combined with mini spin column purification

Applications

  • RNA isolation from cultured cells, animal and plant tissue, yeast, bacteria, and viruses
  • Real-time RT-PCR, Northern blotting, primer extension, array technology, and RNase protection assays

Product Overview—NucleoZOL

Technology One-phase extraction
Format Reagent
Starting Material <1 x 107 cultured cells, bacteria, and yeast
<100 mg human/animal/plant tissue
<0.4 ml (viral) fluids
Fragment Size miRNA (10–200 nt), large RNA (>200 nt)
Typical Yield (total RNA) Liver: 6–8 µg/mg tissue
Kidney, spleen: 3–4 µg/mg tissue
Muscle, brain, lung: 0.5–1.5 µg/mg
Cultured cells: 4–10 µg/106 cells
Typical Yield (large RNA) Liver: 5–7 µg/mg tissue
Kidney, spleen: 3–4 µg/mg tissue
Muscle, brain, lung: 0.5–1.5 µg/mg
Cultured cells: 3–8 µg/106 cells
A260/A280 1.8–2.1
Typical RIN (RNA Integrity Number) >9
Elution Volume Flexible
Preparation Time <1 hr

Product Overview—NucleoSpin RNA Set for NucleoZOL

Technology Silica membrane technology
Format Mini spin columns
Starting Material ≤500 µl NucleoZOL* sample
Fragment Size miRNA (10–200 nt), large RNA (>200 nt)
Typical Recovery 85–95%
Typical RIN (RNA Integrity Number) Depending on sample quality (no significant loss of RIN detected)
Elution Volume 60 µl
Preparation Time <1 hr
Binding Capacity 200 µg

*NucleoZOL not included.


 
 
Products
Offers Cat. # Product Contents Size Price Units Select
740404.200 NucleoZOL™ 200 mL $269.00
740406.50 NucleoSpin® RNA Set for NucleoZOL™ 50 Preps $193.00
 

 

 
 

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