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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  RNA >  Total_RNA_from_Cells_and_Tissues >  Miniprep

RNA Isolation or Purification—Mini Spin Columns

NucleoSpin RNA is recommended for isolation and purification of total RNA from cultured cells, tissue, bacteria, yeast, cell-free biological fluids, and reaction mixtures without the use of organic compounds (e.g., Trizol). This kit allows purification of up to 70 µg of highly pure, DNA-free total RNA.

Total RNA prepared with NucleoSpin RNA is suitable for applications such as reverse transcriptase-PCR (qRT-PCR), Northern blotting, primer extension, array technology, and RNase protection assays. For isolation of high-quality RNA, it is important to prevent degradation of the RNA and to eliminate genomic DNA. With the NucleoSpin RNA method, cells are lysed in the presence of chaotropic salts. The lysis buffer immediately inactivates RNases, which are present in virtually any biological material, and creates appropriate conditions for RNA to bind to the silica membrane. After lysis, homogenization and reduction of viscosity are achieved by filtration with NucleoSpin Filter units (shredders), which are provided with the kit. Residual genomic DNA is efficiently removed by on-column digestion with rDNase, which is also included in the kit.

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Features

  • Complete purification kit: DNase I and shredders are included
  • No genomic DNA contamination
  • Yields up to 70 µg of high-quality, ready-to-use RNA without the use of organic compounds (e.g., Trizol)
  • Simple spin column protocol (no need to learn a new protocol)
  • Better performance and outstanding savings
  • Columns and buffers are available separately
  • Cat. No. 740955.240C is designed for use with the QIAcube only

Applications

Kit for isolation and purification of total RNA from cultured cells and tissue (standard protocol), as well as paraffin-embedded tissue, bacterial cells, yeast cells, biological fluids, and samples stored in RNAlater (support protocols) without the use of organic compounds (e.g., Trizol).

Obtain purified RNA suitable for:

  • Real-time RT-PCR
  • Northern blotting
  • Primer extension
  • Array technology
  • RNase protection assays

Product Overview

Technology Silica membrane
Format Mini spin columns
Starting Material <5 x 106 cultured cells
<109 bacterial cells
<30 mg tissue
up to 108 yeast cells
Fragment Size >200 b
Typical Yield14–70 µg
Elution Volume 40–100 µl
Preparation Time 30 min/6 preps

 
 
Products
Cat. # Product Contents Size Price Units Select
740955.250 NucleoSpin® RNA 250 Preps $1,072.00
740955.50 NucleoSpin® RNA 50 Preps $233.00
740955.240C NucleoSpin® RNA 240 Preps $1,072.00
 

 

 
 

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