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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  RNA >  Total_RNA_from_Cells_and_Tissues >  Midiprep

Complete Midiprep Kit for Purification of Total RNA—NucleoSpin RNA Midi

NucleoSpin RNA Midi allows isolation of DNA-free, high-quality RNA from large amounts of starting material such as cultured cells, tissue, bacteria, and yeast without the use of organic compounds (e.g., Trizol). The working procedure is similar to standard RNA purification methods and includes effective rDNase digestion of contaminating DNA during the purification process.

At-A-Glance Documents Images & Data Resources

Features

  • Complete midi kit with rDNase for on-column digestion—includes shredders
  • Purify total RNA from cells, tissue, and yeast
  • Ready-to-use RNA for performance in any downstream applications
  • No use of toxic material such as phenol/chloroform (e.g., Trizol), CsCl, or ethanol

Applications

Isolate total RNA from cultured cells and tissue (standard protocol), as well as bacterial cells, yeast cells, and samples stored in RNAlater (support protocols).

Purified RNA suitable for:

  • Real-time RT-PCR
  • Northern blotting
  • Primer extension
  • Array technology
  • RNase protection assays

Product Overview

Technology Silica membrane
Format Midi spin columns
Starting Material

< 5 x 107 cultured cells
< 1010 bacterial cells
200 mg tissue
< 3 x 108 yeast cells

Fragment Size 200 bp–20 kb
Typical Yield 180 µg from 107 HeLa cells
620 µg from 4 x 107 HeLa cells
A260/280 1.90–2.10
Elution Volume 500 µl
Preparation Time 80 min/4 preps

 
 
Products
Cat. # Product Package Size Price # of Units Select
740962.20 NucleoSpin® RNA Midi 20 Preps $238.00
 

 

 

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