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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  RNA >  Total_RNA_from_Cells_and_Tissues >  96-Well_Plates

High-Throughput Total RNA Isolation & Purification—NucleoSpin 96 RNA

NucleoSpin 96 RNA is designed for automated isolation and purification of total RNA from animal or human cell cultures and tissue. With the NucleoSpin 96 RNA method, cells are lysed in presence of chaotropic salts. The lysis buffer immediately inactivates RNases, which are present in virtually any biological material, and creates appropriate conditions for RNA to bind to the silica membrane. Genomic DNA is removed by performing on-column DNA digestion using the rDNase provided in the kit. Ready-to-run protocols are available and can be customized to fit your needs. NucleoSpin 96 RNA provides consistent RNA quality, reproducible yields of RNA, and high-throughput procedures free of cross-contamination without the use of organic extraction (e.g., Trizol). These kits are powerful tools for such applications as microarray analysis in respect to gene expression profiling, and drug discovery.

The kits allow isolation and purification of total RNA from up to 107 cells or up to 30 mg tissue (centrifuge processing). Contaminating DNA, which is also bound to the silica membrane, is removed by on-column DNA digestion using DNase I (DNase I is supplied with the kits). Salts, metabolites, and macromolecular cellular components are removed by simple washing steps with three different buffers. Pure RNA is finally eluted under low ionic strength conditions with RNase-free water (pH 8.0–8.5).

At-A-Glance Documents Images & Data Resources

Features

  • High-throughput total RNA isolation and purification: 96-well plates
  • Fast and convenient sample processing with rDNase included in the kits
  • Reproducible yields for a wide variety of samples
  • Suitable for manual and automated processing, under vacuum or centrifugation
  • Innovative MN wash plate minimizes risk of cross-contamination
  • No organic extraction (e.g., Trizol) or precipitation

Applications

Manual or automated isolation and purification of total RNA from cultured cells and tissue.

Application Notes

The NucleoSpin 8/96 RNA kits are compatible with many major robotic systems. Application notes are available for:

  • Eppendorf epMotion 5075
  • CyBi-RoboSpense
  • X-Tractor Gene
  • AVISO Theonyx
  • Hamilton MICROLAB STAR
  • Tecan Freedom
  • Tecan Genesis / Te-Mo 96
  • MWG RoboSeq 4204

Product Overview

Technology Silica membrane
Format 96-well plates
Processing Manual or automated, vacuum or centrifugation
Starting Material <107 cultured cells
30 mg tissue
Fragment Size 200 b–20 kb
Typical Yield  <100 µg
Elution Volume 50–130 µl
Preparation Time 70 min/plate

 
 
Products
Cat. # Product Package Size Price # of Units Select
740709.4 NucleoSpin® 96 RNA 4 x 96 Preps $1,234.00
740709.2 NucleoSpin® 96 RNA 2 x 96 Preps $695.00
740709.24 NucleoSpin® 96 RNA 24 x 96 Preps $6,685.00
740466.4 NucleoSpin® 96 RNA Core Kit 4 x 96 Preps $1,055.00
740733.4 NucleoSpin® 96 RNA Tissue Core Kit 4 x 96 Preps $1,055.00
 

 

 

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