High-Throughput Total RNA Isolation and Purification—NucleoSpin 8 RNA
NucleoSpin 8 RNA is designed for automated isolation and purification of total RNA from animal or human cell cultures and tissue. With the NucleoSpin 8 RNA method, cells are lysed in presence of chaotropic salts. The lysis buffer immediately inactivates RNases, which are present in virtually any biological material, and creates appropriate conditions for RNA to bind to the silica membrane. Genomic DNA is removed by performing on-column DNA digestion using the rDNase provided in the kit. Ready-to-run protocols are available and can be customized to fit your needs. NucleoSpin 8 RNA provides consistent RNA quality, reproducible yields of RNA, and high-throughput procedures free of cross-contamination without the use of organic extraction (e.g., Trizol). These kits are powerful tools for such applications as microarray analysis in respect to gene expression profiling, and drug discovery.
The kits allow isolation and purification of total RNA from up to 107 cells or up to 30 mg tissue (centrifuge processing). Contaminating DNA, which is also bound to the silica membrane, is removed by on-column DNA digestion using DNase I (DNase I is supplied with the kits). Salts, metabolites, and macromolecular cellular components are removed by simple washing steps with three different buffers. Pure RNA is finally eluted under low ionic strength conditions with RNase-free water (pH 8.0–8.5).