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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  RNA >  Stool

Isolation of Total RNA from Stool Samples—NucleoSpin RNA Stool

The NucleoSpin RNA Stool kit is designed for efficient isolation of RNA, including small RNA species, from fresh and frozen stool samples. The kit combines NucleoZol with Lysis Buffer RST1 for chemical disruption of stool and associated microbes. The mechanical lysis is performed using NucleoSpin Bead Tubes Type A (containing ceramic beads) in combination with a mechanical disruption device. No enzymatic reactions, such as protease digestion, are required to homogenize the sample material.

Undissolved sample material and the ceramic beads are subsequently removed by a short centrifugation. A subsequent lysate clearing step comprises addition of Binding Buffer RST2 followed by a short centrifugation. The supernatant is finally cleared by passing it through a NucleoSpin Inhibitor Removal Column, which removes substances in stool samples that interfere with qPCR. Binding conditions are adjusted by adding additional Binding Buffer RST2 to the flowthrough of the NucleoSpin Inhibitor Removal Column. Next, the sample is loaded onto a NucleoSpin RNA Stool Column. Residual contaminants such as complex polysaccharides, bile salts, and other PCR inhibitors are removed by an efficient washing procedure using Buffer RST2, as well as Wash Buffers RST3, RST4, and RST5. In between the different washing steps, residual DNA is removed via an on-column DNA digestion step using rDNase. After a drying step, ready-to-use RNA can be eluted with RNase-free water.

  At-A-Glance   Documents

Features

  • Speedy isolation of total RNA from various stool specimens
  • Combination of mechanical homogenization and chemical lysis ensures optimized RNA yield, including small RNA
  • Isolated RNA does not show any PCR inhibition due to NucleoSpin Inhibitor Removal Columns
  • Suitable for various human and animal stool samples

Applications

  • RNA isolation from stool samples
  • Typical downstream applications: real-time RT-PCR, northern blotting, primer extension, array technology, RNase protection assays

Product Overview

Technology Silica membrane technology combined with NucleoSpin Bead Tubes Type A
Format Mini spin columns
Sample Material
Fresh or frozen stool samples
Fragment Size
≥18 nt
Typical Yield
10–30 µg
A260/2801.9–2.1
A260/230 1.8–2.5
Typical RIN (RNA Integrity Number)
>7.5
Elution Volume 100 µl
Preparation Time 70 min/10 preps
Binding Capacity
200 µg

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
New Cat. # Product Contents Size Price Units Select
740130.10 NucleoSpin® RNA Stool 10 Preps $115.00
740130.50 NucleoSpin® RNA Stool 50 Preps $440.00
 

 

 
 

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