The NucleoBond RNA Soil kit is designed for the purification of RNA from soil and sediment samples. In combination with the DNA Set for NucleoBond RNA Soil, RNA and DNA can be isolated from the same soil sample. Purified nucleic acids are suitable for metagenomic studies, for example, when the genome and transcriptome are analyzed from the same sample.
Most soils contain relatively low amounts of RNA, compared to DNA. Therefore, the kit was designed with proven NucleoBond technology to enable high sample input and yield pure nucleic acids. Sample lysis using our NucleoSpin Bead Tubes Type A in the presence of the optimized Lysis Buffer E1 and phenol:chloroform:isoamyl alcohol ensures efficient sample disruption. The optional Buffer OPT enables efficient RNA and DNA purification from mineralic and organic soil samples.