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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  RNA >  Soil

Isolation of RNA from Soil—NucleoBond RNA Soil

The NucleoBond RNA Soil kit is designed for the purification of RNA from soil and sediment samples. In combination with the DNA Set for NucleoBond RNA Soil, RNA and DNA can be isolated from the same soil sample. Purified nucleic acids are suitable for metagenomic studies, for example, when the genome and transcriptome are analyzed from the same sample.

Most soils contain relatively low amounts of RNA, compared to DNA. Therefore, the kit was designed with proven NucleoBond technology to enable high sample input and yield pure nucleic acids. Sample lysis using our NucleoSpin Bead Tubes Type A in the presence of the optimized Lysis Buffer E1 and phenol:chloroform:isoamyl alcohol ensures efficient sample disruption. The optional Buffer OPT enables efficient RNA and DNA purification from mineralic and organic soil samples.

  At-A-Glance   Documents   Images & Data

Features

  • Anion exchange technology to optimize RNA yield and purity
  • Ideal for metagenomic studies
  • Combination of mechanical homogenization and chemical lysis allows processing of large sample amounts
  • Parallel preparation of RNA and DNA in one hour

Applications

  • RNA isolation from soil for metagenomic analysis
  • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays

Product Overview

Technology Gravity flow columns combined with NucleoSpin Bead Tubes Type A
Starting material <2 g of soil
Fragment size 100 nt
Typical yield 1–10 µg
A260/A280 1.7–2.1
A260/A230 1.8–2.5
Typical RIN (RNA integrity number) >8.5
Elution volume 100 µl
Preparation time 60 min/6 preps
Technology Gravity flow columns combined with NucleoSpin Bead Tubes Type A

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.



 
 
Products
New Cat. # Product Contents Size Price Units Select
740140.20 NucleoBond® RNA Soil 20 Preps $357.00
740141.20 DNA Set for NucleoBond® RNA Soil 20 Preps $71.00
 

 

 
 

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