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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  RNA >  RNA_Clean-Up

RNA Clean-Up Mini Spin Columns for Small Samples—NucleoSpin RNA Clean-Up

The NucleoSpin RNA Clean-up kits provide a simple, fast, and convenient way to clean up your RNA from prepurified RNA (phenol/chloroform), enzymatic reactions such as amplification and labeling reactions, DNase digestions, and more. The kit offers complete removal of RT-PCR inhibitors, leading to superior RT-PCR results. The time-saving procedure is based on the RNA purification kits (NucleoSpin RNA II) without the DNase digestion and homogenization steps.

NucleoSpin RNA Clean-up is also recommended for RNA isolation from small amounts of cultured cells whenever copurification of some genomic DNA is acceptable. For isolating RNA from cells and tissue with the least DNA contamination, we recommend rDNase-containing NucleoSpin RNA kits. The kits allow purification of pure RNA with an A260/280 ratio generally exceeding 1.9 (measured in TE buffer, pH 7.5).

The purified RNA is ready to use for such applications as enzymatic labeling reactions (including dye incorporation), reverse transcriptase-PCR (RT-PCR), and DNA/RNA-based chip hybridizations. The NucleoSpin RNA Clean-up kit can also be used to clean up in vitro-transcribed RNA and aminoallyl mRNA, as well as biotinylated and fluorescently-labeled RNA.

At-A-Glance Documents Images & Data Resources

Features

  • Complete removal of RT-PCR inhibitors
  • Time-saving procedure based on NucleoSpin RNA II, without DNase digestion and homogenization steps
  • RNA clean-up from prepurified RNA (phenol/chloroform), enzymatic reactions (e.g., amplification reactions, labeling reactions, DNase digestions)
  • Competitive price per prep

Applications

RNA clean-up of:

  • Prepurified RNA (e.g. Trizol)
  • Reaction mixtures
  • Aminoallyl mRNA
  • Biotinylated RNA
  • RNA isolation from up to 105 cultured cells (whenever copurification of some genomic DNA is acceptable, since the kit does not contain rDNase)

RNA ready to use for:

  • Enzymatic labeling reactions
  • RT-PCR
  • DNA/RNA-based chip hybridizations

Product Overview

Technology Silica membrane
Format Mini spin columns
Starting Material Up to 200 µl phenol/chloroform extract or reaction mixture
Fragment Size >200 b
Typical Recovery 85–95%
A260/280 1.90–2.10
Elution Volume 40–100 µl
Preparation Time 20 min/6 preps
Binding Capacity 200 µg

 
 
Products
Cat. # Product Package Size Price # of Units Select
740948.50 NucleoSpin® RNA Clean-up 50 Preps $179.00
740948.250 NucleoSpin® RNA Clean-up 250 Preps $856.00
740903.10 NucleoSpin® RNA Clean-up XS 10 Preps $61.00
740903.50 NucleoSpin® RNA Clean-up XS 50 Preps $267.00
740903.250 NucleoSpin® RNA Clean-up XS 250 Preps $1,184.00
 

 

Recommended Products
Cat. # Product Package Size Price # of Units Select
740963 rDNase Set Each $63.00
 
 


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