The NucleoSpin RNA Clean-up kits provide a simple, fast, and convenient way to clean up your RNA from prepurified RNA (phenol/chloroform), enzymatic reactions such as amplification and labeling reactions, DNase digestions, and more. The kit offers complete removal of RT-PCR inhibitors, leading to superior RT-PCR results. The time-saving procedure is based on the RNA purification kits (NucleoSpin RNA II) without the DNase digestion and homogenization steps.
NucleoSpin RNA Clean-up is also recommended for RNA isolation from small amounts of cultured cells whenever copurification of some genomic DNA is acceptable. For isolating RNA from cells and tissue with the least DNA contamination, we recommend rDNase-containing NucleoSpin RNA kits. The kits allow purification of pure RNA with an A260/280 ratio generally exceeding 1.9 (measured in TE buffer, pH 7.5).
The purified RNA is ready to use for such applications as enzymatic labeling reactions (including dye incorporation), reverse transcriptase-PCR (RT-PCR), and DNA/RNA-based chip hybridizations. The NucleoSpin RNA Clean-up kit can also be used to clean up in vitro-transcribed RNA and aminoallyl mRNA, as well as biotinylated and fluorescently-labeled RNA.