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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  RNA >  RNA-DNA-Protein >  RNA-DNA

NucleoBond RNA/DNA—Isolation & Purification of RNA & DNA from the Same Sample

NucleoBond RNA/DNA 80 and 400 kits are designed for the isolation and purification of up to 80 or 400 µg ultra-pure total RNA respectively, plus a separate genomic DNA fraction. A special manufacturing process guarantees RNase-free handling. These kits contain NucleoBond AXR columns (anion-exchange columns for the highest purity and yield) and all of the required buffers. Due to the resolving power of NucleoBond anion exchange resin, contaminants are washed away, and RNA and genomic DNA can be eluted separately.

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Features

  • Extra-high purity for up to 400 µg total RNA—via anion exchange chromatography
  • Separate isolation of different RNA species (tRNA, rRNA, mRNA)
  • Separate elution of genomic DNA

Applications

  • Isolate and purify total RNA and genomic DNA from cultured cells, tissue, and bacteria.

Product Overview

NucleoBond RNA/DNA 80

NucleoBond RNA/DNA 400

Technology

Anion exchange chromatography

Anion exchange chromatography

Format

Midi gravity-flow columns

Maxi gravity-flow columns

Starting Material

5 x 106 cultured eukaryotic cells
20 mg tissue
0.5 x 108 bacteria/yeast cells

2 x 107 cultured eukaryotic cells
100 mg tissue
2 x 109 bacteria/yeast cells

Fragment Size

50 bp–300 kb

50 bp–300 kb

Typical Yield

70 µg from 5 x 106 cultured cells
30 µg from 20 mg tissue
50 µg from 0.5 x 108 bacteria/yeast cells

300 µg from 2 x 107 cultured cells
150 µg from 100 mg tissue
200 µg from 2 x 109 bacteria/yeast cells

A260/280

1.80–1.95

1.80–1.95

Preparation Time

1.5–2.5 hr

1.5–2.5 hr

Binding Capacity

80 µg

400 µg


 
 
Products
Cat. # Product Contents Size Price Units Select
740651 NucleoBond® RNA/DNA 400 10 Preps $113.00
740650 NucleoBond® RNA/DNA 80 25 Preps $171.00
 

 

 
 

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