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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  RNA >  Plant

Mini Spin Kit for Isolation & Purification of Total RNA from Plants—NucleoSpin RNA Plant

NucleoSpin RNA Plant is designed for isolation and purification of DNA-free, high-quality RNA from a wide variety of plant and fungal samples. The working procedure is similar to the standard RNA purification method without the use of organic compounds (e.g., Trizol)—removal of contaminating DNA is achieved by effective rDNase digestion during the purification process.

The NucleoSpin RNA Plant kit features 2 alternative lysis buffers: in most cases, use of buffer RA1 is recommended for lysis due to its strong denaturing properties. The presence of peculiar metabolites in a variety of plant tissues or fungi, however, may lead to solidification of the lysate, resulting in a non-processible slurry. In such cases, the alternative buffer RAP is the buffer of choice. For thorough homogenization and reduction of lysate viscosity, NucleoSpin Filter Columns are also provided with the kit.

At-A-Glance Documents Images & Data Resources

Features

  • Complete mini kit includes DNase I and shredders
  • No genomic DNA contamination
  • Optimal lysis conditions for all plant species—two alternative lysis buffers
  • No use of organic compounds (e.g., Trizol)
  • Yields up to 70 µg of high-quality, ready-to-use RNA
  • Simple spin column protocol (no need to learn new protocol)
  • Better performance and outstanding savings

Applications

Isolate and purify total RNA from plant cells and tissue, and filamentous fungi without the use of organic compounds (e.g., Trizol).

Purified RNA is suitable for:

  • Real-time RT-PCR
  • Northern blotting
  • Primer extension
  • Array technology
  • RNase protection assays

Product Overview

Technology Silica membrane
Format Mini spin columns
Starting Material 1–100 mg tissue
Fragment Size 200 bp–20 kb
Typical Yield 3–70 µg from 100 mg plant material
Elution Volume 60 µl
Preparation Time 30 min/6 preps

 
 
Products
Cat. # Product Package Size Price # of Units Select
740949.50 NucleoSpin® RNA Plant 50 Preps $275.00
740949.250 NucleoSpin® RNA Plant 250 Preps $1,255.00
740949.10 NucleoSpin® RNA Plant 10 Preps $62.00
 

 

 

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