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Products >  Nucleic_Acid_Purification >  RNA >  FFPE

Isolation and Purification of Total RNA from FFPE Samples

The NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS kits provide a fast, efficient, and convenient method for isolating both small (e.g., miRNA) and large RNA from formalin-fixed, paraffin-embedded (FFPE) tissue.

NucleoSpin totalRNA FFPE includes mini spin columns (30–50 µl elution volume) to purify standard FFPE samples (<50 mg tissue), while NucleoSpin totalRNA FFPE XS includes XS columns (5–30 µl elution volume) to purify very small FFPE samples (< 5 mg tissue, e.g., microdissections). Both kits include blue-colored Paraffin Dissolver for easy visualization of phase separation and convenient paraffin removal, without using toxic organic solvents such as xylene—and rDNase for efficient on-column DNA removal. The kits combine decrosslinking and tissue lysis in one step, providing reliable yields of high-quality total RNA for enhanced RT-PCR performance. The NucleoSpin totalRNA FFPE XS kit makes it possible to isolate highly concentrated RNA suitable for qRT-PCR from even trace amounts of FFPE samples. 

At-A-Glance Documents Images & Data Resources

Features

  • Efficient isolation of total RNA from FFPE samples
  • Two kit options—high recovery from standard and very small FFPE samples
  • Convenient paraffin removal—no xylene required
  • Enhanced RT-PCR performance 

Applications

  • Rapid isolation of RNA from formalin-fixed, paraffin-embedded samples, e.g.,
    • Colon carcinoma (unstained or hematoxylin-stained)
    • Colon mucosa
    • Adenocarcinoma of the colon transversum
    • Liver (rat/human)
    • Liver carcinoma (hematoxylin-stained)
    • Lymph node
    • Spleen (rat/human)
    • Pancreas (rat/human; diabetic/non-diabetic)
    • Placenta
  • Isolation of RNA from fresh and archived FFPE samples
  • Isolation of RNA from specimens on object slides (stained or unstained)
  • Typical downstream application: RT-PCR

Product Overview

NucleoSpin totalRNA FFPE

NucleoSpin totalRNA FFPE XS

Technology

Silica membrane

Silica membrane

Format

Mini spin columns

XS spin columns

Starting Material

4–10 sections (10 µm) of
up to 6 mm2 total area
<50 mg tissue

1–3 sections (10 µm) of
up to 6 mm2 total area
<5 mg tissue

Typical Yield

Depends on the amount and
quality of the sample

Depends on the amount and
quality of the sample

Elution Volume

30–50 µl

5–30 µl

Preparation Time

70 min/6 preps (90 min including
optional rDNase digest)

70 min/6 preps (90 min including
optional rDNase digest)

Binding Capacity

200 µg

100 µg


 
 
Products
New Cat. # Product Package Size Price # of Units Select
740982.10 NucleoSpin® totalRNA FFPE 10 Preps $72.00
740982.50 NucleoSpin® totalRNA FFPE 50 Preps $329.00
740982.250 NucleoSpin® totalRNA FFPE 250 Preps $1,439.00
  740969.10 NucleoSpin® totalRNA FFPE XS 10 Preps $79.00
  740969.50 NucleoSpin® totalRNA FFPE XS 50 Preps $349.00
  740969.250 NucleoSpin® totalRNA FFPE XS 250 Preps $1,529.00
  740968.25 Paraffin Dissolver 25 mL $26.00
 

 

 

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