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Nucleic Acid Purification

Products >  Nucleic_Acid_Purification >  RNA >  Blood >  HTP

High-throughput total RNA isolation from blood—NucleoSpin 96 RNA Blood

The NucleoSpin 96 RNA Blood kit combines the stringency of guanidine-isothiocyanate lysis with effective digestion of genomic DNA by rDNase treatment and the speed of silica membrane technology. This kit offers consistent RNA quality, reproducible yields of RNA, and high-throughput procedures free of cross-contamination. The NucleoSpin 96 RNA Blood kit provides a powerful tool for microarray analysis in gene expression profiling, drug discovery, and molecular diagnostics applications. The kit allows purification of total RNA from up 400 µl whole blood, and consists of components designed mainly for vacuum processing. For centrifugation, suitable consumables for waste collection must be ordered separately (see ”Consumables supplied by user” below). In the NucleoSpin 96 RNA Blood procedure, blood cells are directly lysed by incubation in a solution containing large amounts of chaotropic ions. This lysis buffer immediately inactivates RNases and creates appropriate binding conditions which favor adsorption of RNA to the silica membrane of the NucleoSpin 96 RNA Blood Binding Plate.

Contaminating DNA, which is also bound to the silica membrane, is efficiently removed by on-column DNA digestion. A 15-min digestion at room temperature is sufficient, due to the highly specific activity of the rDNase. Salts, metabolites, and macromolecular cellular components are removed by simple washing steps with three different buffers. Pure RNA is eluted under low ionic strength conditions with RNase-free water.

  At-A-Glance   Documents


  • Simple, direct total blood lysis at room temperature
  • Compatible with common blood collection tubes and anticoagulants (e.g., EDTA, citrate, and heparin)
  • Suitable for fresh and frozen blood samples
  • rDNase included for efficient removal of genomic DNA
  • Suitable for manual and automated processing, under vacuum or centrifugation
  • Innovative MN Wash Plate minimizes risk of cross-contamination


  • Manual or automated isolation of total RNA from fresh or frozen whole blood stabilized with, for example, EDTA, citrate, or heparin
  • Typical downstream applications: qRT-PCR, next-generation sequencing, blotting, array technology

* Kits to be used for research purposes only.

Product Overview

Technology Silica membrane technology
Format 96-well plates
Processing Manual or automated, vacuum or centrifugation
Starting material <400 µl whole blood (fresh or frozen)
Fragment size >200 nt
Typical yield 1–8 µg (400 µl blood)*
Typical RNA quality (A260/A280) 1.9–2.1
Elution volume 50–130 µl
Preparation time 100 min/plate
Binding capacity 100 µg

* RNA yield strongly depends on the leukocyte number in each individual blood sample.

New Cat. # Product Contents Size Price Units Select
740225.2 NucleoSpin® 96 RNA Blood 2 x 96 Preps $818.00
740225.4 NucleoSpin® 96 RNA Blood 4 x 96 Preps $1,451.00



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