Clontech
login | register | order now | view cart | feedback
Products
Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  RNA >  Blood

Mini & Midi Spin Kits for Isolation of Total RNA from Blood—NucleoSpin RNA Blood & NucleoSpin RNA Blood Midi

The NucleoSpin RNA Blood and NucleoSpin RNA Blood Midi kits provide a simple, efficient, and direct total blood lysis method for isolating high-quality RNA from fresh or frozen whole blood that has been stabilized with anticoagulants (e.g., EDTA, citrate, or heparin). The entire procedure is performed at room temperature, with no need for selective erythrocyte lysis at 4°C (a common and cumbersome requirement for other methods). The isolated RNA is suitable for downstream applications such as qRT-PCR, next-generation sequencing, blotting, and array technology.

NucleoSpin RNA Blood

The NucleoSpin RNA Blood kit allows isolation of high-quality RNA from up to 400 µl of fresh or frozen whole blood. After total blood cell lysis in Lysis Buffer DL, the RNA is bound to a NucleoSpin RNA Blood Binding Column. Contaminating DNA and inhibitors are efficiently removed by on-column rDNase digestion and washes with two different wash buffers. The purified RNA is then eluted with RNase-free water.

Compared with competitor kits, NucleoSpin RNA Blood kits show higher yields from smaller sample volumes. In addition, it is possible to obtain a linear increase in yield relative to sample volume. Both fresh and frozen blood samples can be used to purify RNA of comparable yield and quality.

NucleoSpin RNA Blood Midi

The NucleoSpin RNA Blood Midi kit allows isolation of high-quality RNA from up to 1,300 µl of fresh or frozen whole blood. The working procedure is similar to the NucleoSpin RNA Blood method and includes simple, efficient, and direct total blood lysis, as well as effective rDNase digestion of contaminating DNA during the purification process. No selective erythrocyte lysis is needed and the whole procedure can be performed at room temperature.

At-A-Glance Documents Images & Data

Features

  • Simple, direct total blood lysis at room temperature
  • Convenient handling of different sample volumes and types
  • Superior RNA yield and quality from up to 400 µl (or 400–1,300 µl for Midi kits) of whole blood
  • Efficient on-column DNA removal provides increased sensitivity in downstream applications
  • Compatible with common blood collection tubes and anticoagulants (e.g., EDTA, citrate, and heparin)

Applications

  • qRT-PCR
  • Next-generation sequencing
  • Blotting
  • Array technology

Product Overview

NucleoSpin RNA Blood

NucleoSpin RNA Blood Midi

Technology

Silica membrane

Silica membrane

Format

Mini spin columns

Midi spin columns

Starting Material

<400 µl whole blood
(fresh or frozen)

400–1,300 µl whole blood

Fragment Size

>200 nt

>200 nt

Typical Yield

1–8 µg (400 µl whole blood)*

4–26 µg (1,300 µl whole blood)*

A260/280

1.9–2.1

1.9–2.1

Elution Volume

40–120 µl

200–400 µl

Preparation Time

55 min/6 preps

75 min/6 preps

Binding Capacity

200 µg

700 µg

*RNA yield strongly depends on the leukocyte number in each individual blood sample.


 
 
Products
Cat. # Product Package Size Price # of Units Select
740200.50 NucleoSpin® RNA Blood 50 Preps $249.00
740210.20 NucleoSpin® RNA Blood Midi 20 Preps $218.00
 

 

 

Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions