The NucleoSpin RNA Blood and NucleoSpin RNA Blood Midi kits provide a simple, efficient, and direct total blood lysis method for isolating high-quality RNA from fresh or frozen whole blood that has been stabilized with anticoagulants (e.g., EDTA, citrate, or heparin). The entire procedure is performed at room temperature, with no need for selective erythrocyte lysis at 4°C (a common and cumbersome requirement for other methods). The isolated RNA is suitable for downstream applications such as qRT-PCR, next-generation sequencing, blotting, and array technology.
NucleoSpin RNA Blood
The NucleoSpin RNA Blood kit allows isolation of high-quality RNA from up to 400 µl of fresh or frozen whole blood. After total blood cell lysis in Lysis Buffer DL, the RNA is bound to a NucleoSpin RNA Blood Binding Column. Contaminating DNA and inhibitors are efficiently removed by on-column rDNase digestion and washes with two different wash buffers. The purified RNA is then eluted with RNase-free water.
Compared with competitor kits, NucleoSpin RNA Blood kits show higher yields from smaller sample volumes. In addition, it is possible to obtain a linear increase in yield relative to sample volume. Both fresh and frozen blood samples can be used to purify RNA of comparable yield and quality.
NucleoSpin RNA Blood Midi
The NucleoSpin RNA Blood Midi kit allows isolation of high-quality RNA from up to 1,300 µl of fresh or frozen whole blood. The working procedure is similar to the NucleoSpin RNA Blood method and includes simple, efficient, and direct total blood lysis, as well as effective rDNase digestion of contaminating DNA during the purification process. No selective erythrocyte lysis is needed and the whole procedure can be performed at room temperature.