Home Takara Clontech Logo Takara Clontech
login | register | order now | view cart | feedback
Products
Nucleic Acid Purification

Products >  Nucleic_Acid_Purification >  Plasmid >  Miniprep >  Gravity

NucleoBond PC 20—Plasmid Miniprep

NucleoBond PC 20 plasmid miniprep kits allow you to purify plasmid DNA in just a few hours without phenol/chloroform extraction. These kits can be used to purify high- and low-copy-number plasmids, cosmids, BACs, PACs, and YACs grown from bacterial cultures. The resulting plasmids are at least as pure as plasmids isolated by traditional cesium chloride procedures and can be used for the most demanding molecular biology applications, including mammalian transfection, in vitro transcription, and automated sequencing.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Isolate highly pure plasmid DNA for transfection or sequencing
  • Rapid purification procedure using gravity flow
  • Clear lysates without centrifugation (filters provided with the kit)
  • Highly reproducible purification results

Applications

  • Transfecting sensitive cells
  • Sequencing
  • Cloning
  • PCR
  • Restriction analysis

Product Overview

Technology Anion exchange
Format Mini
Lysate Clarification Centrifugation
Starting Material 1–5 ml (high-copy-number)
3–10 ml (low-copy-number)
Vector Size <300 kb
Typical Yield 3–20 µg
A260/280 1.80–1.95
Preparation Time 60 min/4–6 preps

 
 
Products
Cat. # Product Contents Size Price Units Select
740571 NucleoBond® PC 20 20 Preps $123.00
740571.100 NucleoBond® PC 20 100 Preps $492.00
 

 

Recommended Products
Cat. # Product Size Price Units Select
740562 NucleoBond® Small Rack Each $51.00
740563 NucleoBond® Large Rack Each $74.00
740601 NucleoBond® Buffer Set I Each $62.00
 
 
 

Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions