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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  Plasmid >  Miniprep >  EasyPure

Plasmid Miniprep (Ultra-Fast): NucleoSpin Plasmid EasyPure Kit

NucleoSpin Plasmid EasyPure is a high-speed and high-yield miniprep kit which combines the highest plasmid yield with a very fast procedure. The NucleoSpin Plasmid EasyPure Columns supplied in the kit provide a binding capacity of 35 µg plasmid DNA and allow reliable yields, even from higher culture volumes. Rapid purification is achieved by using a combined washing and drying step, which allows 6 minipreps to be completed within 14 min.

This simple procedure starts with alkaline lysis of a bacterial culture. The blue LyseControl indicator reagent premixed in the Lysis Buffer allows you to visualize completion of neutralization and alkaline lysis by turning colorless. The lysate is then cleared by centrifugation and the supernatant is loaded onto a NucleoSpin Plasmid EasyPure Column. Contaminants such as salts, metabolites, nucleases, and soluble macromolecular cellular components are removed by a single washing and drying step.

At-A-Glance Documents Images & Data

Features

  • Ultra-fast, small-scale preparation of up to 35 µg of high-purity plasmid DNA
  • LyseControl allows visualization of completed alkaline lysis
  • Liquid RNase A provides easy handling—no need to dissolve enzyme
  • One combined washing and drying step enables ultra-fast purification (6 minipreps in 14 min)

Applications

  • High-copy plasmids from E. coli
  • Low-copy plasmids, P1 constructs, or cosmids (increased buffer volumes required)
  • Plasmid DNA from Gram-positive bacteria and plasmid DNA clean-up from reaction mixtures
  • Typical downstream applications: cloning, sequencing, PCR, transformation, restriction analysis

Product Overview

  
Technology Silica membrane
Format Mini spin columns
Starting Material 2–10 ml E. coli culture
Vector Size <15 kb
Typical Yield 15–30 µg
A260/280 1.80–1.85
Elution Volume 50 µl
Preparation Time 14 min/6 preps
Binding Capacity 35 µg 

 
 
Products
New Cat. # Product Package Size Price # of Units Select
740727.50 NucleoSpin® Plasmid EasyPure 50 Preps $54.00
740727.250 NucleoSpin® Plasmid EasyPure 250 Preps $223.00
 

 

 

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