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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  Plasmid >  Miniprep >  96-Well

Plasmid DNA Purification—Manual Processing 96-Well Plates—NucleoSpin 96 Plasmid

The NucleoSpin 96 Plasmid Kits are designed for purifying up to 20 µg of high-copy plasmids from 1–5 ml E. coli overnight culture, preferably grown in LB medium. The NucleoSpin Plasmid Binding Strips/Plates contain a unique silica membrane that binds DNA in the presence of chaotropic salt. 96-well plates can be processed manually, under vacuum, or by centrifugation.

Vacuum Processing

The NucleoSpin 96 Plasmid Kits can be used manually with the NucleoVac 96 vacuum manifold. Additionally, a suitable centrifuge is required for sample preparation steps.

Centrifugation

Centrifuge processing requires a microtiterplate centrifuge which can accommodate the NucleoSpin 96 Plasmid Plate stacked on a Round- or Square-well Block and reaches accelerations of 5,600–6,000 x g (bucket height: 85 mm). For further information about suitable centrifuges, please contact Technical Support.

At-A-Glance Documents Images & Data Resources

Features

  • Time-saving parallel isolation of plasmid DNA
  • Processes up to 5 ml bacterial culture
  • NucleoSpin 96 Filter Plate included, for convenient filtration of bacterial lysates
  • Processing can be done under vacuum or by centrifugation
  • Innovative MN Wash Plate minimizes risk of cross-contamination
  • DNA ready to use for PCR, Southern blotting, any kind of enzymatic reaction

Applications

  • PCR
  • Southern blotting
  • Any kind of enzymatic reaction

Product Overview

Technology Silica membrane
Format 96-well plates
Processing Manual, vacuum, or centrifugation
Lysate Clarification 96-well filter plate
Starting Material 1–5 ml E. coli culture
Vector Size <15 kb
Typical Yield 4–6 µg/ml E. coli culture
A260/280 1.70–1.85
Elution Volume 75–150 µl
Preparation Time 45 min/plate
Binding Capacity 20 µg

 
 
Products
Cat. # Product Package Size Price # of Units Select
740625.24 NucleoSpin® 96 Plasmid 24 x 96 Preps $4,202.00
740625.4 NucleoSpin® 96 Plasmid 4 x 96 Preps $840.00
740625.1 NucleoSpin® 96 Plasmid 1 x 96 Preps $231.00
 

 

 

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