The NucleoSpin 96 Plasmid Kits are designed for purifying up to 20 µg of high-copy plasmids from 1–5 ml E. coli overnight culture, preferably grown in LB medium. The NucleoSpin Plasmid Binding Strips/Plates contain a unique silica membrane that binds DNA in the presence of chaotropic salt. 96-well plates can be processed manually, under vacuum, or by centrifugation.
The NucleoSpin 96 Plasmid Kits can be used manually with the NucleoVac 96 vacuum manifold. Additionally, a suitable centrifuge is required for sample preparation steps.
Centrifuge processing requires a microtiterplate centrifuge which can accommodate the NucleoSpin 96 Plasmid Plate stacked on a Round- or Square-well Block and reaches accelerations of 5,600–6,000 x g (bucket height: 85 mm). For further information about suitable centrifuges, please contact Technical Support.