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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  Plasmid >  Midiprep >  NucleoBond_Xtra_Midi

Midipreps for Plasmid DNA Purification—NucleoBond Xtra Midi/Midi Plus

NucleoBond Xtra Midiprep kits are a new generation of anion exchange midiprep plasmid purification kits based on the patented NucleoBond technology. The typical yields of ≥ 250 μg of ultra-pure plasmid DNA can be obtained in about half of the time compared to other Midiprep kits. The entire plasmid purification procedure takes 68 min with NucleoBond Xtra Midi kits and 28 min with NucleoBond Xtra Midi Plus kits.

NucleoBond Xtra Midiprep kits contain an enlarged column with a lower silica resin bed. This in turn enables faster flow of lysate and buffers through the columns. Specially-designed column filters are included for convenient and time-saving clarification of bacterial lysates. The column filters are supplied inserted in the NucleoBond Xtra Midiprep columns and allow parallel clarification of bacterial lysate and loading onto the column. Their large, structured surface leads to high filter flow rates and minimizes the risk of clogging. The improved silica material has greater DNA binding capacity and is based on the proven NucleoBond anion-exchanger group MAE (methylaminoethanol). Optimized buffer compositions additionally lead to improved alkaline lysis and increased column flow rates.

At-A-Glance Documents Images & Data Resources

Features

  • Xtra fast—reduce prep time by up to 60%
  • Xtra high yields—obtain up to 100% more transfection-grade DNA, typical yield: 250 µg
  • Xtra purity—obtain the highest purity via proven anion exchange technology
  • Xtra flexible—choose from Midiprep/Maxiprep, with or without NucleoBond Finalizer (for ultra-fast purification)

Applications

  • Transfecting sensitive cells
  • Sequencing
  • Cloning

Product Overview

NucleoBond Xtra Midi

NucleoBond Xtra Midi Plus

Technology

Anion exchange

Anion exchange

Format

Midi

Midi

Lysate Clarification

Column filters

Column filters

Starting Material

<200ml (high-copy-number)
<400 ml (low-copy-number)

<200 ml(high-copy-number)
<400 ml (low-copy-number)

Vector Size

<300 kb

<300 kb

Typical Yield

>250 µg

>250 µg

A260/280

1.80–1.95

1.80–1.95

Isopropanol Precipitation

Centrifugation

NucleoBond Finalizer

Preparation Time

68 min/4 preps

28 min/4 preps


Procedure

Bacteria are harvested from an overnight culture and lysed by an optimized alkaline lysis procedure. The bacterial lysate is cleared and loaded onto the equilibrated column in one step, and the plasmid DNA binds to the anion-exchange resin. The first washing step (using equilibration buffer) is performed with the column filter inserted, to wash out residual lysate from the filter and obtain maximum recovery of DNA. After washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation (NucleoBond Xtra Midiprep kit) or by using the NucleoBond Finalizer included in the NucleoBond Xtra Midiprep Plus kits to reduce the total prep time to about 30 minutes.


 
 
Products
Cat. # Product Package Size Price # of Units Select
740410.10 NucleoBond® Xtra Midi 10 Preps $94.00
740410.100 NucleoBond® Xtra Midi 100 Preps $823.00
740410.50 NucleoBond® Xtra Midi 50 Preps $442.00
740420.10 NucleoBond® Xtra Midi EF 10 Preps $141.00
740420.50 NucleoBond® Xtra Midi EF 50 Preps $649.00
740412.10 NucleoBond® Xtra Midi Plus 10 Preps $114.00
740412.50 NucleoBond® Xtra Midi Plus 50 Preps $523.00
740422.10 NucleoBond® Xtra Midi Plus EF 10 Preps $177.00
740422.50 NucleoBond® Xtra Midi Plus EF 50 Preps $765.00
 

 

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Cat. # Product Package Size Price # of Units Select
740415 NucleoBond® Xtra Combi Rack Each $73.00
740417 NucleoBond® Xtra Buffer Set I Each $50.00
740427 NucleoBond® Xtra EF Buffer Set I Each $53.00
 
 

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