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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  Plasmid >  Midiprep >  NucleoBond_PC_100

NucleoBond PC 100—Plasmid Midiprep

NucleoBond PC 100 plasmid midiprep kits allow you to purify plasmid DNA in just a few hours without phenol/chloroform extraction. These kits can be used to purify high- and low-copy-number plasmids, cosmids, BACs, PACs, and YACs grown from bacterial cultures. The resulting plasmids are at least as pure as plasmids isolated by traditional cesium chloride procedures and can be used for the most demanding molecular biology applications, including mammalian transfection, in vitro transcription, and automated sequencing.

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Features

  • Isolate highly pure plasmid DNA for transfection or sequencing
  • Rapid purification procedure using gravity flow
  • Clear lysates without centrifugation (filters provided with the kit)
  • Highly reproducible purification results

Applications

  • Transfecting sensitive cells
  • Sequencing
  • Cloning
  • PCR
  • Restriction analysis

Product Overview

Technology Anion exchange
Format Midi
Lysate Clarification Folded filters
Starting Material 5–30 ml (high-copy-number)
10–100 ml (low-copy-number)
Vector Size <300 kb
Typical Yield 20–100 µg
A260/280 1.80–1.95
Preparation Time 65 min/4–6 preps

 
 
Products
Cat. # Product Contents Size Price Units Select
740573.100 NucleoBond® PC 100 100 Preps $794.00
740573 NucleoBond® PC 100 20 Preps $176.00
 

 

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