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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  Plasmid >  Megaprep

NucleoBond PC 2000—Megaprep Plasmid DNA

NucleoBond PC 2000 plasmid megaprep kits allow you to purify up to 2,000 µg plasmid DNA in just a few hours without phenol/chloroform extraction. The kits can be used to purify high- and low-copy-number plasmids, cosmids, BACs, PACs, and YACs grown from bacterial cultures. The resulting plasmids are at least as pure as plasmids isolated by traditional cesium chloride procedures and can be used for the most demanding molecular biology applications, including mammalian transfection, in vitro transcription, and automated sequencing.

At-A-Glance Documents Images & Data Resources

Features

  • Isolate highly pure plasmid DNA for transfection or sequencing
  • Rapid purification procedure using gravity flow
  • Clear lysates without centrifugation (filters provided with the kit)
  • Highly reproducible purification results

Applications

  • Transfecting sensitive cells
  • Sequencing
  • Cloning
  • PCR
  • Restriction analysis

Product Overview

Technology Anion exchange
Format Mega
Lysate Clarification Folded filters
Starting Material 150–2,000 ml (high-copy-number)
Vector Size <300 kb
Typical Yield 1,700–2,000 µg
A260/280 1.80–1.95
Preparation Time 90–120 min/4–6 preps

 
 
Products
Cat. # Product Package Size Price # of Units Select
740576 NucleoBond® PC 2000 5 Preps $220.00
 

 

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