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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  Plasmid >  Maxiprep >  Nucleobond_PC_500

NucleoBond PC 500—Plasmid Maxiprep

NucleoBond PC 500 plasmid maxiprep kits allow you to purify plasmid DNA in just a few hours without phenol/chloroform extraction. High-copy-number and low-copy-number plasmids, cosmids, BACs, PACs, and YACs grown from bacterial cultures can be purified using these kits. The resulting plasmids are at least as pure as plasmids isolated by traditional cesium chloride procedures and can be used for the most demanding molecular biology applications, including mammalian transfection, in vitro transcription, and automated sequencing.

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Features

  • Isolate highly pure plasmid DNA for transfection or sequencing
  • Rapid purification procedure using gravity flow
  • Clear lysates without centrifugation (filters provided with the kit)
  • Highly reproducible purification results

Applications

  • Transfection of sensitive cells
  • Sequencing
  • Cloning
  • PCR
  • Restriction analysis

Product Overview

Technology Anion Exchange
Format Maxi
Lysate Clarification Folded filters
Starting Material 30–150 ml (high-copy number)
100–500 ml (low-copy number)
Vector Size <300 kb
Typical Yield 450–520 µg
A260/280 1.80–1.95
Preparation Time 80–90 min/4 preps

 
 
Products
Cat. # Product Contents Size Price Units Select
740574 NucleoBond® PC 500 10 Preps $186.00
740574.100 NucleoBond® PC 500 100 Preps $1,668.00
740574.25 N Application Error
 
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