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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  Plasmid >  Maxiprep >  NucleoBond_Xtra_Maxi

Maxipreps for Plasmid DNA Purification—NucleoBond Xtra Maxi/Maxi Plus

NucleoBond Xtra Maxi and Maxi Plus are a new generation of anion exchange plasmid purification kits based on the patented NucleoBond technology. The typical yields of >1,000 µg of ultra-pure plasmid DNA can be obtained in about half of the time compared to other Maxi kits based on anion exchange chromatography. The entire plasmid purification procedure takes 80 min with NucleoBond Xtra Maxi and 34 min with NucleoBond Xtra Maxi Plus.

NucleoBond Xtra Filters: Specially designed column filters are supplied inserted in the NucleoBond Xtra columns, allowing a convenient and time-saving parallel clarification of bacterial lysate and loading onto the column—minimizing the risk of clogging.

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for a fast purification. The improved silica material is based on the anion-exchange group MAE.

NucleoBond Xtra Buffers: Optimized buffer compositions lead to improved alkaline lysis and increased column flow rates. Get more DNA in a shorter processing time.

NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits. The NucleoBond Finalizer technology replaces the tedious and time-consuming centrifugation step that follows every isopropanol precipitation of diluted DNA samples.

At-A-Glance Documents Images & Data Resources

Features

  • Xtra fast—reduce prep time by up to 60%
  • Xtra high yields—obtain up to 100% more transfection-grade DNA. Typical yield: 1,000 µg
  • Xtra purity—obtain the highest purity via proven anion exchange technology
  • Xtra flexible—choose from Midiprep/Maxiprep, with or without NucleoBond Finalizer (for ultra-fast purification)

Applications

  • Transfecting sensitive cells
  • Sequencing
  • Cloning

Product Overview

NucleoBond Xtra Maxi

NucleoBond Xtra Maxi Plus

Technology

Anion exchange

Anion exchange

Format

Maxi

Maxi

Lysate Clarification

Column filters

Column filters

Starting Material

< 600 ml (high-copy-number)
< 1,200 ml (low-copy-number)

< 600 ml (high-copy-number)
< 1,200 ml (low-copy-number)

Vector Size

<300 kb

<300 kb

Typical Yield

>1,000 µg

>1,000 µg

A260/280

1.80–1.95

1.80–1.95

Isopropanol Precipitation

Centrifugation

NucleoBond Finalizer

Preparation Time

80 min/4 preps

34 min/4 preps


 
 
Products
Cat. # Product Package Size Price # of Units Select
740414.10 NucleoBond® Xtra Maxi 10 Preps $217.00
740414.100 NucleoBond® Xtra Maxi 100 Preps $1,903.00
740414.50 NucleoBond® Xtra Maxi 50 Preps $1,021.00
740424.10 NucleoBond® Xtra Maxi EF 10 Preps $296.00
740424.50 NucleoBond® Xtra Maxi EF 50 Preps $1,473.00
740416.10 NucleoBond® Xtra Maxi Plus 10 Preps $238.00
740416.50 NucleoBond® Xtra Maxi Plus 50 Preps $1,141.00
740426.10 NucleoBond® Xtra Maxi Plus EF 10 Preps $349.00
740426.50 NucleoBond® Xtra Maxi Plus EF 50 Preps $1,662.00
 

 

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Cat. # Product Package Size Price # of Units Select
740415 NucleoBond® Xtra Combi Rack Each $73.00
740417 NucleoBond® Xtra Buffer Set I Each $50.00
740427 NucleoBond® Xtra EF Buffer Set I Each $53.00
 
 


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