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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  Plasmid >  Endotoxin_Free >  Midiprep

Midiprep Plasmid DNA Purification Endotoxin-Free—NucleoBond Xtra Midi EF/Midi Plus EF

Minimizing the level of endotoxin contamination in purified plasmid DNA is critical for many applications. For this reason, MACHEREY-NAGEL has developed a specific patented procedure that reduces endotoxins to a very low level. Unlike competitor kits, there is no need for a time-consuming incubation step to remove the endotoxins (see procedure). The working procedure for endotoxin-free plasmid preparation is identical to the standard protocol. NucleoBond Xtra EF features up to 68% reduced prep times and up to 100% increased yields of endotoxin-free plasmid DNA, compared to other commercially available kits.

NucleoBond Xtra filters: These specially designed column filters are supplied inserted in the NucleoBond Xtra EF columns. They allow a convenient and time-saving parallel clarification of bacterial lysate and loading onto the column. The large, structured filter surface leads to high filter flow rates and minimizes the risk of clogging.

NucleoBond Xtra columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for a fast purification.

NucleoBond Xtra EF buffers: Optimized buffer compositions lead to improved alkaline lysis, increased column flow rates, highest yields, and most efficient endotoxin removal.

NucleoBond Xtra finalizers: The finalizers, provided with the Plus kits, replace the time-consuming centrifugation step that follows every isopropanol precipitation of diluted DNA samples.

At-A-Glance Documents Images & Data Resources

Features

  • Xtra fast—reduce prep time by up to 68% (100 min for Midi EF and 45 min for Midi Plus EF)
  • Xtra high yields—obtain up to 100% more transfection-grade plasmid DNA, typical yield 250–500 µg
  • Xtra efficient endotoxin removal—obtain DNA with less than 0.05 EU/µg
  • Xtra flexible – with or without NucleoBond Finalizer (for ultrafast purification)

Applications

Purified DNA is suitable for:

  • Transfection of highly sensitive cells
  • Lentivirus production

Product Overview

NucleoBond Xtra Midi EF NucleoBond Xtra Midi Plus EF
Technology

Anion exchange

Anion exchange

Format

Midi gravity flow columns

Midi gravity flow columns

Lysate Clarification

Column filters (included)

Column filters (included)

Starting Material

<200 ml (high copy)
<400 ml (low copy)

<200 ml (high copy)
<400 ml (low copy)

Vector Size

<300 kb

<300 kb

Typical Yield

>250 µg

>250 µg

A260/280

1.80–1.95

1.80–1.95

Endotoxin Level

<0.05 EU/µg DNA

<0.05 EU/µg DNA

Isopropanol Precipitation

Centrifugation

NucleoBond Finalizer

Preparation Time

100 min/4 preps

45 min/4 preps


 
 
Products
Cat. # Product Package Size Price # of Units Select
740420.10 NucleoBond® Xtra Midi EF 10 Preps $141.00
740420.50 NucleoBond® Xtra Midi EF 50 Preps $649.00
740422.10 NucleoBond® Xtra Midi Plus EF 10 Preps $177.00
740422.50 NucleoBond® Xtra Midi Plus EF 50 Preps $765.00
 

 

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Cat. # Product Package Size Price # of Units Select
740415 NucleoBond® Xtra Combi Rack Each $73.00
740427 NucleoBond® Xtra EF Buffer Set I Each $53.00
 
 

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