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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  Plasmid >  BAC >  Maxiprep

BAC Purification—NucleoBond BAC 100

The NucleoBond BAC 100 Kit is designed for purifying large DNA fragments such as cosmids, bacteriophage P1 clones, P1-derived artificial chromosomes (PACs), and bacterial artificial chromosomes (BACs), without phenol/chloroform extraction. The procedure takes less than 1 hour to isolate BAC DNA free from any kind of RNA, proteins, metabolites, dyes, or carbohydrates. After purification, all nucleic acids show absorbance ratios of A260/A280 as high as 1.8–2.0.

Low amounts of impurities, which often act as strong inhibitors for enzymatic processing, sequencing, transfections, transcriptions, etc., are removed completely.

NucleoBond Folded Filters, which are specially designed to eliminate the centrifugation step after the alkaline lysis step, are provided with the kit. Using the filters reduces hands-on-time and eliminates plastic waste common to other filtration systems. In approximately 10 minutes of unattended operation, complete removal of SDS and cellular debris from plasmid samples is achieved. NucleoBond Folded Filters do not induce shearing of large DNA constructs, such as PACs or BACs.

The patented anion exchange resin in the NucleoBond column is known to provide high-purity DNA equivalent or superior to that obtained by two successive rounds of CsCl ultracentrifugation. The plasmid purification procedure is performed in the absence of toxic material such as phenol, chloroform, ethidium bromide, or CsCl.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • For purification of P1 constructs, BACs, PACs, and other large constructs
  • Vectors up to 300 kb can be purified
  • Increased buffer volumes included
  • NucleoBond Folded Filters included for lysate clarification
  • Anion exchange technology

Applications

  • Cloning
  • Sequncing
  • PCR
  • Transformation
  • Restriction analysis

Product Overview

Technology Anion exchange
Format Maxi
Lysate Clarification Folded filters
Starting Material 100–500 ml
Vector Size <300 kb
Typical Yield 10–100 µg
A260/280 1.80–1.95
Preparation Time 120 min/2–4 preps

 
 
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740579 null 10 Preps $276.00
 

 

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