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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  Plasmid >  BAC >  HTP

BAC DNA Isolation in 96-Well Plate Format—NucleoSpin 96 Flash

The NucleoSpin 96 Flash Kit lets you rapidly isolate BAC DNA from bacterial cultures using a high-throughput method. Process samples in a 96-well format using either a vacuum manifold or a tabletop centrifuge. Obtain BAC yields of <1 µg from 1.3–3.9 ml of E. coli culture or up to 8 µg of high-copy-number plasmid from 1.1–1.3 ml of E. coli culture.

At-A-Glance Documents Images & Data Resources

Features

  • Suitable for high-throughtput purification of large constructs (BACs, cosmids)
  • Vectors up to 300 kb can be purified
  • Manual or automated processing

Applications

DNA suitable for:

  • Sequencing
  • PCR
  • Restriction

Product Overview

Technology Alkaline lysis with subsequent filtration and precipitation
Format 96-well plates
Processing Manual or automated, vacuum or centrifugation
Lysate Clarification 96-well plate filter plates
Starting Material1.1–1.3 ml E. coli culture
1.3–3.9 ml E. coli culture (BACs)
Vector Size<250 kb
Typical Yield8 µg from 1.1–1.3 ml E. coli culture (high-copy plasmid)
<1 µg from 1.3–3.9 ml E. coli culture (BACs)
Preparation Time90 min/2 plates

 
 
Products
Cat. # Product Package Size Price # of Units Select
740618.4 NucleoSpin® 96 Flash 4 x 96 Preps $570.00
740618.24 NucleoSpin® 96 Flash 24 x 96 Preps $3,107.00
740618.2 NucleoSpin® 96 Flash 2 x 96 Preps $325.00
 

 

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