Clontech
login | register | order now | view cart | feedback
Products
Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  Plasmid >  BAC >  HTP

BAC DNA Isolation in 96-Well Plate Format—NucleoSpin 96 Flash

The NucleoSpin 96 Flash Kit lets you rapidly isolate BAC DNA from bacterial cultures using a high-throughput method. Process samples in a 96-well format using either a vacuum manifold or a tabletop centrifuge. Obtain BAC yields of <1 µg from 1.3–3.9 ml of E. coli culture or up to 8 µg of high-copy-number plasmid from 1.1–1.3 ml of E. coli culture.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Suitable for high-throughtput purification of large constructs (BACs, cosmids)
  • Vectors up to 300 kb can be purified
  • Manual or automated processing

Applications

DNA suitable for:

  • Sequencing
  • PCR
  • Restriction

Product Overview

Technology Alkaline lysis with subsequent filtration and precipitation
Format 96-well plates
Processing Manual or automated, vacuum or centrifugation
Lysate Clarification 96-well plate filter plates
Starting Material1.1–1.3 ml E. coli culture
1.3–3.9 ml E. coli culture (BACs)
Vector Size<250 kb
Typical Yield8 µg from 1.1–1.3 ml E. coli culture (high-copy plasmid)
<1 µg from 1.3–3.9 ml E. coli culture (BACs)
Preparation Time90 min/2 plates

 
 
Products
Cat. # Product Contents Size Price Units Select
740618.4 NucleoSpin® 96 Flash 4 x 96 Preps $570.00
740618.24 NucleoSpin® 96 Flash 24 x 96 Preps $3,107.00
740618.2 NucleoSpin® 96 Flash 2 x 96 Preps $325.00
 

 

Recommended Products
Offers Cat. # Product Contents Size Price Units Select
740681 NucleoVac™ 96 Vacuum Manifold Each $534.00
  740641 NucleoVac™ Vacuum Regulator Each $158.00
 
 
HTTP/1.1 200 OK Date: Thu, 23 Feb 2017 09:53:16 GMT Connection: close Transfer-Encoding: chunked Content-Type: text/html; charset=iso-8859-1 200 OK

OK

The server encountered an internal error or misconfiguration and was unable to complete your request.

Please contact the server administrator, ohs_admin@paloalto.local and inform them of the time the error occurred, and anything you might have done that may have caused the error.

More information about this error may be available in the server error log.