Magnetic Bead-Based Isolation of Viral RNA or DNA—NucleoMag 96 Virus
NucleoMag 96 Virus is designed for rapid manual and automated small-scale preparation of viral RNA/DNA from cell-free body fluids such as serum or plasma. Typically, 96 samples can be purified in less than 120 minutes on common automation platforms. The kit provides reagents for the purification of viral DNA/RNA from up to 200 µl sample volume.
The NucleoMag product family is based on magnetic bead technology. The binding technology is similar to the silica membrane technology: Nucleic acids are bound to the magnetic beads under chaotropic salt conditions, contaminants are washed away with ethanolic wash buffers, and pure RNA/DNA can be eluted from the beads using elution buffer.
The NucleoMag technology can be easily adapted to common liquid handling instruments. It can be used either with static-pin separators (e.g., NucleoMag SEP) or magnetic separators integrated into robotic workstations.