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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  High-Throughput >  PCR_Clean-Up >  Magnetic

Ultrafiltration & Magnetic PCR Clean-Up 96-Well Plates

The NucleoFast 96 PCR Clean-Up Kit and NucleoFast 96 PCR Plates use ultrafiltration for high recovery of nucleic acids in less than 20 minutes. The nucleic acids from the PCR reaction are collected on the filter membrane while the contaminants are filtered directly to the waste. The NucleoFast protocol is faster because it eliminates the intermediate wash steps required with silica-based purification systems. The 96 PCR Clean-Up Kit includes buffers and elution plate. The sturdy, one-piece plate is easily adapted to automated processing. The purified PCR products are suitable for most common downstream applications, including capillary sequencing, microarray spotting, restriction analysis, labeling, or cloning.

Magnetic PCR Clean-up Kits—96-Well Plates

NucleoMag 96 PCR Kits are magnetic bead-based kits for parallel purification of PCR products, which provide a reliable, cost- and labor-efficient tool. PCR products are selectively bound to the NucleoMag PCR Beads, while contaminants (e.g., primers, dNTPs, enzymes, salts) are washed away. Purified PCR products are eluted from the beads under low-salt conditions and are ready to use in downstream applications such as sequencing or microarray spotting. Since the magnetic bead technology provides a closed system (no transfer of sample from one well to another is necessary during the preparation), there is no risk of cross-contamination. The NucleoMag technology can be easily adapted to common laboratory automation platforms. It can be used either with static-pin separators (e.g., NucleoMag SEP) or magnetic separators integrated into robotic workstations.

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Features

  • Rapid, cost-efficient 96-well ultrafiltration kit for PCR clean-up—NucleoFast 96 PCR
  • Magnetic bead-based PCR clean-up—NucleoMag 96 PCR

Applications

  • Ready-to-use DNA for sequencing and microarray spotting
  • Manual or automated use

Product Overview

NucleoFast 96 PCRNucleoMag 96 PCR
TechnologyUltrafiltration technologyMagnetic bead technology
Format

96-well plates

Highly reactive superparamagnetic
beads (size of beads: 1–3 µm)
ProcessingManual or automatedManual or automated
Starting Material20–300 µl<50 µl PCR reaction
Fragment Size>150 bp150 bp–10 kb
Typical Recovery40–95% 80-95%
A260/2801.70–1.80 1.70-1.90
Recovery Volume25–100 µl 25 µl
Preparation Time20 min/plate (for 25 µl PCR reactions)30–45 min/plate

 
 
Products
Offers Cat. # Product Contents Size Price Units Select
743500.4 NucleoFast® 96 PCR Clean-Up Kit 4 x 96 Preps $477.00
  743100.01 NucleoFast® 96 PCR Plate 1 Plate $50.00
743100.50 NucleoFast® 96 PCR Plates 50 Plates $1,488.00
743100.10 NucleoFast® 96 PCR Plates 10 Plates $426.00
744100.1 NucleoMag® 96 PCR 1 x 96 Preps $107.00
744100.4 NucleoMag® 96 PCR 4 x 96 Preps $372.00
744100.24 NucleoMag® 96 PCR 24 x 96 Preps $2,045.00
 

 

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744900 NucleoMag® SEP Each $580.00
740481 Square-well Block 4 Plates $31.00
740481.24 Square-well Block 24 Plates $183.00
740486.24 Elution Plate, U-bottom 24 Sets $102.00
740641 NucleoVac Vacuum Regulator Each $147.00
 
 
 

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