The NucleoSpin Tissue kits are designed for the rapid purification of highly pure genomic DNA from tissue samples, mouse tails, bacteria, yeast, and forensic samples (hair, dried blood spots, buccal swabs, and cigarette stubs) and Formalin-Fixed, Paraffin-Embedded (FFPE) samples using mini spin columns. Up to 35 µg of high-purity genomic DNA can be prepared (typical yields from tissue or cells: 15–25 µg). The obtained DNA can be used directly for PCR, Southern blotting, or any kind of enzymatic reaction.
With the NucleoSpin Tissue method, lysis is achieved by incubation of the samples in a solution containing SDS and proteinase K at 56°C. Appropriate conditions for binding of DNA to the silica membrane of the NucleoSpin Tissue Columns are created by addition of large amounts of chaotropic ions and ethanol to the lysate. The binding process is reversible and specific to nucleic acids. Contaminants are removed by efficient washing with buffer. Pure genomic DNA is eluted under low ionic strength conditions in a slightly alkaline elution buffer, and is ready to use for subsequent reactions.
Genomic DNA Isolation from Extremely Small Tissue Samples
The NucleoSpin Tissue XS kits are designed to provide maximum recovery and concentration of highly pure genomic DNA from Xtra Small (extremely dilute and low-volume) tissue samples—such as microdissected tissue, biopsies (e.g., fine needle aspiration), dried blood spots, buccal swabs, forensic samples, FFPE samples, and other small samples. NucleoSpin Tissue XS overcomes the limitations of conventional kits with an innovative column that is optimized for very small samples, allowing efficient elution in only 5–30 µl and resulting in highly concentrated DNA.
The NucleoSpin Tissue XS Column contains a funnel-shaped thrust ring which holds a silica membrane of small diameter, allowing the accurate dispensing of small volumes on the membrane. NucleoSpin Tissue XS allows isolation of genomic DNA from samples as small as 0.025–10 mg of microdissected tissue, as little as 1–30 µl fresh, frozen or stabilized blood, and as few as 10–10,000 cells. The isolated DNA is free of impurities and suitable for sensitive downstream applications.