Clontech
login | register | order now | view cart | feedback
Products
Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  Genomic_DNA >  Tissue >  Single_Prep_Gravity

Isolation of Genomic DNA from Tissues—NucleoBond AXG

NucleoBond AXG columns are anion exchange columns for isolating ultra-pure genomic DNA from tissue, bacteria, or yeast. MACHEREY-NAGEL offers buffer sets for extracting genomic DNA from tissue and bacteria, which include all required buffers and enzymes (proteinase K and RNase A). Using these buffer sets in combination with NucleoBond AXG columns allows isolation of up to 500 µg genomic DNA from different matrices. Isolation of genomic DNA from yeast requires additional buffers (see user manual). NucleoBond AXG columns are available in three different sizes (AXG 20, AXG 100, and AXG 500).

The quality of the isolated genomic DNA is extraordinarily high. AXG columns run by gravity flow yield high molecular weight DNA without shearing. Samples are first lysed with proteinase K. Before loading, the sample is diluted with buffer N2 in order to guarantee a good flow rate as well as quantitative DNA binding. After efficient washing, the genomic DNA is eluted with high-salt buffer and precipitated with isopropanol. The DNA purified using this method is suitable for all kinds of subsequent reactions.

At-A-Glance Documents Images & Data Resources

Features

  • Ultra-pure genomic DNA from from tissue, bacteria, or yeast
  • Combine NucleoBond AXG columns and the corresponding buffer set for highest flexibility:
  • NucleoBond AXG columns + NucleoBond Buffer Set III for isolation of genomic DNA from bacteria and yeast
  • NucleoBond AXG columns + NucleoBond Buffer Set IV for isolation of genomic DNA from tissue

Applications

Isolation of genomic DNA from bacteria, yeast and tissue

Product Overview

NucleoBond AXG 20

NucleoBond AXG 100

NucleoBond AXG 500

Technology

Anion exchange technology

Format

Mini gravity-flow columns

Midi gravity-flow columns

Maxi gravity-flow columns

Starting 
Material

2–5 ml bacterial culture
3–5 ml yeast culture
<20 mg tissue

15–20 ml bacterial culture
10–20 ml yeast culture
<100 mg tissue

60–80 ml bacterial culture
100–500 ml yeast culture
<400 mg tissue

Fragment Size

500 bp–300 kb

500 bp–300 kb

500 bp–300 kb

Typical Yield

20 µg

100 µg

500 µg

A260/280

1.80–1.95

1.80–1.95

1.80–1.95

Preparation
Time

4–5 hr

4–5 hr

4–5 hr

Binding Capacity

20 µg

100 µg

500 µg


 
 
Products
Cat. # Product Package Size Price # of Units Select
740545 NucleoBond® AXG 100 20 Columns $191.00
740544 NucleoBond® AXG 20 20 Columns $134.00
740546 NucleoBond® AXG 500 10 Columns $213.00
740603 NucleoBond® Buffer Set III Each $128.00
740604 NucleoBond® Buffer Set IV Each $126.00
 

 

Recommended Products
Cat. # Product Package Size Price # of Units Select
740562 NucleoBond® Small Rack Each $51.00
740563 NucleoBond® Large Rack Each $74.00
 
 

Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions