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Nucleic Acid Purification

Products >  Nucleic_Acid_Purification >  Genomic_DNA >  Tissue >  Magnetic

Magnetic Bead-Based Isolation of Genomic DNA from Tissue—NucleoMag 96 Tissue

NucleoMag 96 Tissue is designed for the manual or automated small-scale preparation of genomic DNA from human and animal tissue, cultured cells, or bacteria, in a 96-well format. After lysis of the sample, genomic DNA is selectively bound to the NucleoMag Beads. Contaminants are washed away and the DNA can be eluted from the beads under low-salt conditions. Since the magnetic bead technology provides a closed system (no transfer of sample from one well to another is necessary during the preparation), the risk for cross contamination is reduced.

Typically, 96 samples can be purified in less than 120 minutes on common automation platforms. The kit provides reagents for the purification of 10–20 µg genomic DNA from samples (e.g., 20 mg tissue or 107 cells). The purified DNA is ready to use for PCR, Southern blotting, and any enzymatic reaction.

At-A-Glance Documents Images & Data Resources

Features

  • Minimize cross-contamination with a one-tube procedure
  • Small elution volumes—as low as 50 µl
  • Suitable for manual and automated processing
  • Flexible—complete processing possible at room temperature (excluding lysis step)

Applications

  • Manual or automated isolation of DNA from human and animal tissue, cultured cells, or bacteria

Product Overview

Technology Magnetic bead technology
Format Highly reactive superparamagnetic beads
(size of beads: 1–3 µm)
Processing Manual or automated
Starting Material <20 mg tissue, <107 cells or bacteria
Fragment Size 300 bp– >30 kb
Typical Yield 10–20 µg
A260/280 >1.6 to 1.9
Elution Volume >50 µl
Preparation Time 120 min/96 preps
Binding Capacity 0.4 µg/1 µl beads

 
 
Products
Cat. # Product Package Size Price # of Units Select
744300.1 NucleoMag® 96 Tissue 1 x 96 Preps $268.00
744300.4 NucleoMag® 96 Tissue 4 x 96 Preps $975.00
744300.24 NucleoMag® 96 Tissue 24 x 96 Preps $5,317.00
 

 

 

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