Magnetic Bead-Based Isolation of Genomic DNA from Tissue—NucleoMag 96 Tissue
NucleoMag 96 Tissue is designed for the manual or automated small-scale preparation of genomic DNA from human and animal tissue, cultured cells, or bacteria, in a 96-well format. After lysis of the sample, genomic DNA is selectively bound to the NucleoMag Beads. Contaminants are washed away and the DNA can be eluted from the beads under low-salt conditions. Since the magnetic bead technology provides a closed system (no transfer of sample from one well to another is necessary during the preparation), the risk for cross contamination is reduced.
Typically, 96 samples can be purified in less than 120 minutes on common automation platforms. The kit provides reagents for the purification of 10–20 µg genomic DNA from samples (e.g., 20 mg tissue or 107 cells). The purified DNA is ready to use for PCR, Southern blotting, and any enzymatic reaction.