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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  Genomic_DNA >  Soil

Soil DNA Isolation—NucleoSpin Soil

NucleoSpin Soil for isolation of total DNA from soil microorganisms includes two alternative lysis buffers and an additional Enhancer SX, which can be combined with both lysis buffers. The resulting four different lysis systems make the kit suitable for all types of different samples, which is important because soil is very heterogeneous in pH and amounts of organic or inorganic matter such as humic substances or polysaccharides. The silica membrane in NucleoSpin Soil Columns is optimized for quantitative DNA binding. NucleoSpin Bead Tubes, containing strong ceramic beads, allow efficient lysis of microorganisms in the sample. NucleoSpin Inhibitor Removal Columns are included for convenient elimination of contaminants.

NucleoSpin Soil can process up to 500 mg (wet weight) of starting material. Depending on the individual sample, typical yields are in the range of 2 to 10 µg DNA. The eluted DNA is ready to use (undiluted) for subsequent reactions such as PCR, restriction analysis, etc.

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Features

  • Extract DNA from soil, sludge, and sediment
  • Four lysis options accommodate a variety of samples
  • Designed for isolation of DNA from gram-positive bacteria, archaea, fungi, and algae present in soil, sludge, or sediment
  • Kit includes ready to use Bead Tubes with ceramic beads
  • Isolated DNA is free of PCR inhibitors

Applications

  • Extract total DNA from microorganisms in soil and sediment, and free DNA in soil
  • Quantify and detect microorganisms from environmental samples
  • DNA is suitable for Southern blotting, array technology, RT-PCR, and more

Product Overview

Technology Silica membrane
Format Mini spin columns
Starting Material <500 mg soil, sludge, or sediment
Typical Yield 2–10 µg
Elution Volume30–100 µl
Preparation Time<90 min (10 preps)
Binding Capacity50 µg

 
 
Products
Cat. # Product Contents Size Price Units Select
740780.10 NucleoSpin® Soil 10 Preps $62.00
740780.50 NucleoSpin® Soil 50 Preps $240.00
740780.250 NucleoSpin® Soil 250 Preps $1,071.00
 

 

 
 

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