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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  Genomic_DNA >  RapidLyse >  Single_Prep_Spin

Rapid extraction of genomic DNA—NucleoSpin DNA RapidLyse

The isolation of genomic DNA (gDNA) from most organs is a challenging task. Organs are composed of different tissues with various types of cells and matrices. Large molecules such as collagens and proteoglycans can impede lysis and inhibit downstream enzymatic reactions such as PCR. The new NucleoSpin DNA RapidLyse kit uses a unique lysis chemistry to release gDNA from organs and cells with the highest efficiency.

  At-A-Glance   Documents

Features

  • Unique lysis chemistry efficiently releases gDNA from cells, tissues, and organs
  • Effective lysis in one hour or less
  • Superior gDNA yields compared to standard extraction methods
  • Also available in a 96-well plate format

Applications

  • Rapid isolation of genomic DNA from tail and ear clippings, organs, and cells

Product Overview

Technology Silica membrane technology
FormatMini spin columns
Starting material Fresh, frozen, dried, or ethanol-preserved organs
Eukaryotic cells
Tail or ear clippings
Sample amount Up to 40 mg fresh weight, 1 x 106 cells
Typical yield Up to 4 µg of DNA per mg tissue
A260/280 1.7–1.9
Elution volume 60–100 µl
Lysis time  60 min or less
Preparation time 25 min/6 preps (excluding lysis)
Binding capacity 60 µg

 
 
Products
New Cat. # Product Contents Size Price Units Select
740100.10 NucleoSpin® DNA RapidLyse 10 Preps $24.00
740100.50 NucleoSpin® DNA RapidLyse 50 Preps $99.00
740100.250 NucleoSpin® DNA RapidLyse 250 Preps $445.00
 

 

 
 

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