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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  Genomic_DNA >  Plasma

Purification of Circulating DNA from Plasma—NucleoSpin Plasma XS

Increased amounts of circulating DNA in plasma have been found in a variety of disorders including cancer, autoimmune diseases, infections, certain fetal diseases, trauma, and stroke. Circulating DNA is known to be highly fragmented and low in concentration, which makes it difficult to purify.

NucleoSpin Plasma XS is designed to isolate fragmented DNA larger than 50 bp from human EDTA blood plasma. The kit overcomes the limitations of conventional kits (large membrane diameter associated with large default elution volumes, and a resulting DNA concentration which is too low to be directly used as PCR template). NucleoSpin Plasma XS allows elution in only 5–20 µl, resulting in highly concentrated DNA!

  At-A-Glance   Documents   Images & Data   Resources

Features

  • High recovery of fragments >50 bp
  • Elution in only 5 µl
  • Concentrated DNA even from dilute samples
  • DNA is ready to use for real-time PCR

Applications

  • Purify circulating DNA from plasma or serum
  • Analyze fetal DNA from maternal plasma
  • Detect tumor markers in plasma

Product Overview

Technology Silica membrane
Format XS spin columns
Starting Material 240 µl plasma or serum
Fragment Size >50 bp
Typical Yield 0.1–100 ng per ml plasma, depending on sample
Typical Concentration 1.2–12,000 pg/µl
Elution Volume 5–20 µl
Preparation Time

High sensitivity procedure: 22–27 min/6 preps
Rapid procedure: 15–20 min/6 preps

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price Units Select
740909.25 NucleoSpin® Plasma F 25 Columns $202.00
740900.50 NucleoSpin® Plasma XS 50 Preps $170.00
740900.250 NucleoSpin® Plasma XS 250 Preps $780.00
740900.10 NucleoSpin® Plasma XS 10 Preps $30.00
 

 

 
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