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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  Genomic_DNA >  Plasma >  NucleoSpin_HTP

Isolation of cell-free DNA from plasma—NucleoSpin 96 DNA Plasma

The NucleoSpin 96 DNA Plasma kit is designed for the parallel isolation of cell-free DNA (cfDNA) from up to 2 ml of blood plasma in a 96-well format. After lysis of the plasma sample, cfDNA is effectively bound to a silica membrane without carrier RNA. Elution may be performed under vacuum without the risk of cross-contamination. The design of the NucleoSpin Plasma Binding Plate and the option of performing the complete procedure under vacuum allow the NucleoSpin 96 DNA Plasma kit to easily be adapted to common liquid handling instruments for fully automated processing without any manual steps.

Vacuum processing

The NucleoSpin 96 DNA Plasma kit can be used manually with the NucleoVac 96 Vacuum Manifold. The kit is suitable for automation on laboratory platforms with standard 96-well plate vacuum chambers.

  At-A-Glance   Documents

Features

  • High-throughput solution for cfDNA isolation
  • Manual or automated processing
  • Optimized protocol for Cell-Free DNA BCT (“Streck Tubes”)
  • NucleoSpin 96 DNA Plasma Core Kit: Kit with basic content focused on automation platforms. Additional accessories can be combined as needed.

Applications

  • Typical downstream applications: NGS, qPCR, ddPCR
  • Highly efficient recovery of cell-free DNA from “liquid biopsies”

Product Overview

Technology Silica membrane technology
Format96-well plates
Starting material0.5─2 ml plasma
Blood draw tubes EDTA, Cell-Free DNA BCT (Streck)
Fragment size ≥50 bp
Typical yieldDepends on sample source, storage, and quality
Elution volume 100 µl (70 µl final eluate volume)
Preparation time90 min/96 preps (EDTA plasma)

 
 
Products
New Cat. # Product Contents Size Price Units Select
740873.1 NucleoSpin® 96 DNA Plasma 96 Preps $598.00
740873.4 NucleoSpin® 96 DNA Plasma 4 Plates $1,571.00
740874.1 NucleoSpin® 96 DNA Plasma Core Kit 96 Preps $408.00
740874.4 NucleoSpin® 96 DNA Plasma Core Kit 4 x 96 Preps $1,343.00
 

 

 
 

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