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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  Genomic_DNA >  Plant >  Single_Prep

Isolation of Genomic DNA from Plant—NucleoSpin Plant II & NucleoSpin Plant Midi/Maxi

NucleoSpin Plant II is a next-generation kit designed for the rapid isolation of genomic DNA from plant cells and tissue, with higher yield and quality. The silica membrane in the NucleoSpin Plant II columns is optimized to improve DNA binding. Since plants are very heterogenous and contain many different metabolites, such as polyphenols, polysaccharides, or acidic components, NucleoSpin Plant II offers two different lysis procedures for optimal processing of various samples. This kit includes two optimized, alternative lysis buffers based on the established CTAB and SDS lysis methods.

This kit allows you to process <100 mg of plant tissue (wet weight) or <20 mg plant tissue (dry weight) with yields typically ranging from 1–30 µg genomic DNA. The purified DNA is ready for use in subsequent reactions such as PCR, Southern blotting, and restriction analysis.

Medium- & Large-Scale Genomic DNA Isolation from Plant Tissue

The NucleoSpin Plant Midi and Plant Maxi Kits are designed for isolating genomic DNA from medium to large samples of plant tissue. These kits also include two optimized, alternative lysis buffers, based on the established CTAB and SDS lysis methods, for optimal processing of various sample types.

Starting samples sizes range up to 250 mg (wet weight) for the Plant Midi Kit, and up to 1 g (wet weight) for the Plant Maxi Kit. Typical yields range from 20 to 80 µg of DNA for NucleoSpin Plant Midi, and 60 to 260 µg of DNA for NucleoSpin Plant Maxi, depending on the size and source of the sample. The purified DNA is ready for use in subsequent reactions such as PCR, Southern blotting, and restriction analysis.

At-A-Glance Documents Images & Data Resources

Features

NucleoSpin Plant II

  • Two alternative lysis buffers included for optimal processing of various samples and highest flexibility:
    • Lysis Buffer PL1, based on CTAB lysis method
    • Lysis Buffer PL2, based on SDS lysis method
  • NucleoSpin Filters included for clarification of lysate
  • Improved buffer compositions for higher yield and purity
  • Optimized silica membrane for improved DNA binding
  • RNase A included

NucleoSpin Plant Midi & Maxi

  • Two alternative lysis buffers included for optimal processing of various samples and highest flexibility
    • Lysis Buffer C1, based on CTAB lysis method
    • Lysis Buffer CL, based on SDS lysis method
  • NucleoSpin Filters L included for clarification of lysate
  • RNase A included

Applications

Isolation of DNA from:

  • Plant cells
  • Plant tissue
  • Fungi

Obtained DNA is suitable for:

  • PCR
  • Southern blotting
  • Enzymatic reactions

Product Overview

NucleoSpin
Plant II
NucleoSpin
Plant Midi
NucleoSpin
Plant Maxi
Technology silica membranesilica membranesilica membrane
Format mini spin columnsmidi spin columnsmaxi spin columns
Starting Material <100 mg plant tissue
(wet weight)
<20 mg plant tissue
(dry weight)
<250 mg plant tissue
(wet weight)
<1,000 mg plant tissue
(wet weight)
Lysate Clarification NucleoSpin FiltersNucleoSpin Plant L
Filters
NucleoSpin Plant XL
Filters
Fragment Size 50 bp– >30 kb50 bp– >30 kb50 bp– >30 kb
Typical Yield 1–30 µg20–80 µg60–260 µg
A260/280 1.6–1.91.8–1.91.8–1.9
Elution Volume 2 x 50 µl200 µl500–2,000 µl
Preparation Time 30 min/prep75 min/prep90 min/prep

 
 
Products
Cat. # Product Package Size Price # of Units Select
740770.50 NucleoSpin® Plant II 50 Preps $155.00
740770.250 NucleoSpin® Plant II 250 Preps $669.00
740772.10 NucleoSpin® Plant II Maxi 10 Preps $101.00
740771.20 NucleoSpin® Plant II Midi 20 Preps $120.00
 

 

 

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