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Nucleic Acid Purification

Products >  Nucleic_Acid_Purification >  Genomic_DNA >  Microbial

Isolation of genomic DNA from bacteria, yeast, and fungi—NucleoSpin Microbial DNA

The NucleoSpin Microbial DNA kit is designed for rapid purification of highly pure genomic DNA from microorganisms (gram-negative and gram-positive bacteria, yeast, and fungi). The kit utilizes NucleoSpin Bead Tubes Type B (glass beads; included in the kit) in combination with Liquid Proteinase K, and a common disruption device, e.g., a swing mill, to lyse different sample types using a single procedure. The procedure is fast, simple and provides reliably high DNA yields from a wide range of microbial samples. NucleoSpin Bead Tubes can also be ordered separately if needed.

Appropriate conditions for DNA binding to NucleoSpin Microbial DNA Columns are achieved by addition of large amounts of chaotropic salts (Binding Buffer MG) to the lysate. Contaminants are removed by two efficient washing steps. The DNA is then eluted with a slightly alkaline buffer and is ready to use for subsequent reactions.

  At-A-Glance   Documents


  • Suitable for a large variety of starting materials
  • NucleoSpin Bead Tubes for efficient lysis included—compatible with many cell disruption devices
  • Fast and easy procedure—Liquid Proteinase K included, no additional enzyme required


  • Total DNA from microbial cultures
  • Typical downstream applications: PCR, real-time PCR, southern blotting, enzymatic reactions

Product Overview

Technology Silica membrane technology combined with
NucleoSpin Bead Tubes Type B
Format Mini spin columns
Starting material <40 mg wet weight cell pellet
Fragment size 200 bp–approximately 50 kb
Typical yield Depends on sample type and disruption method
Approximately 5–25 µg (30 mg wet weight cell pellet)
A260/A280 1.6–2.0
Elution volume 100–200 µl
Preparation time 35 min/prep
Binding capacity 60 µg

New Cat. # Product Contents Size Price Units Select
740235.10 NucleoSpin® Microbial DNA 10 Preps $46.00
740235.50 NucleoSpin® Microbial DNA 50 Preps $193.00


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740813.50 NucleoSpin® Bead Tubes Type C 50 Preps $77.00
740469 MN Bead Tube Holder Each $34.00

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