The NucleoSpin Microbial DNA kit is designed for rapid purification of highly pure genomic DNA from microorganisms (gram-negative and gram-positive bacteria, yeast, and fungi). The kit utilizes NucleoSpin Bead Tubes Type B (glass beads; included in the kit) in combination with Liquid Proteinase K, and a common disruption device, e.g., a swing mill, to lyse different sample types using a single procedure. The procedure is fast, simple and provides reliably high DNA yields from a wide range of microbial samples. NucleoSpin Bead Tubes can also be ordered separately if needed.
Appropriate conditions for DNA binding to NucleoSpin Microbial DNA Columns are achieved by addition of large amounts of chaotropic salts (Binding Buffer MG) to the lysate. Contaminants are removed by two efficient washing steps. The DNA is then eluted with a slightly alkaline buffer and is ready to use for subsequent reactions.