NucleoSpin DNA Trace is designed for the preparation of highly pure genomic DNA from small amounts of any tissue, cell, or forensic sample. The NucleoSpin Funnel Columns (NucleoSpin DNA Trace F Columns) included in the kit are ideally suited for collecting traces of nucleic acids from large volumes. The optimized shape of the NucleoSpin Funnel Column allows the combination of a large volume capacity and a small binding membrane diameter. The DNA isolated by NucleoSpin DNA Trace is ready to use for common downstream applications such as PCR, real-time PCR, or other enzymatic reactions.
The small diameter of the silica membrane allows elution volumes as low as 50 to 100 µl (of low-salt buffer or water), leading to highly enriched samples. 8 to 12 NucleoSpin Funnel Columns can be processed in parallel, depending on the centrifuge rotor used. The risk of cross-contamination is eliminated by placing the funnel columns into 50 ml polyethylene tubes which can be closed with screw caps, resulting in self-contained systems. For elution, 0.5 ml elution tubes are fitted directly to the outlet of the funnel columns (collection tubes and elution tubes are provided with the kit).