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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  Genomic_DNA >  Forensic >  Magnetic

Magnetic Bead-Based Isolation of Genomic DNA from Forensic Samples—NucleoMag 96 Trace

NucleoMag 96 Trace is designed for the purification of DNA from forensic samples (e.g., buccal swabs, dried blood spots, cigarette filters). After lysis of the sample, genomic DNA is selectively bound to the NucleoMag Beads. Contaminants are washed away and the DNA can be eluted from the beads under low-salt conditions. Since the magnetic bead technology provides a closed system (no transfer of sample from one well to another is necessary during the preparation), the risk of cross-contamination is reduced. The NucleoMag technology can be easily adapted to common laboratory automation platforms. It can be used either with static-pin separators (e.g., NucleoMag SEP) or magnetic separators integrated into robotic workstations. For more information on adapting any NucleoMag product to your workstation, please contact your local distributor or MN directly.

Typically, 96 samples can be purified in less than 120 minutes on common automation platforms. The kit typically yields 1–3 µg genomic DNA in an elution volume of >50 µl at a concentration of 10–30 ng/µl. The purified DNA is ready to use for PCR, Southern blotting, and any enzymatic reactions.

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Features

  • One-tube procedure minimizes risk of cross-contamination
  • Small elution volumes >50 µl are possible
  • Easily adapted to automated use

Applications

Manual or automated isolation of DNA from:

  • Buccal swabs
  • Dried blood spots
  • Cigarette filters

Purified DNA is ready for:

  • PCR
  • STR analysis
  • Enzymatic reactions

Product Overview

Technology Magnetic bead technology
Format Highly reactive superparamagnetic beads (size of beads: 1–3 µm)
Processing Manual or automated
Starting Material Buccal swabs, dried blood spots, cigarette filters
Typical Yield 1–3 µg
Typical Concentration 10–30 ng/µl
A260/280 >1.6–1.9
Elution Volume >50 µl
Preparation Time <120 min/96 preps
Binding Capacity 0.4 µg/1 µl beads

 
 
Products
Cat. # Product Contents Size Price Units Select
744600.1 NucleoMag® 96 Trace 1 x 96 Preps $219.00
744600.4 NucleoMag® 96 Trace 4 x 96 Preps $798.00
744600.24 NucleoMag® 96 Trace 24 x 96 Preps $4,276.00
 

 

 
 

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