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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  Genomic_DNA >  Forensic >  HTP

High-Throughput Isolation of Genomic DNA from Forensic Samples—NucleoSpin 8/96 Trace

NucleoSpin 8/96 Trace kits are designed for the preparation of highly pure genomic DNA from small amounts of any tissue, cell, or forensic sample (e.g., buccal swabs, blood spots, cigarette filters). The obtained DNA can be used directly as template for PCR. Lysis is achieved by incubation of samples in a solution containing chaotropic ions in the presence of proteinase K. If subsequent separation of the lysate from the sample material is difficult, the lysate can be separated from the forensic material under vacuum or centrifugation using the NucleoSpin Trace Filter Plate (not included in the kits). Appropriate conditions for binding of DNA to the silica membrane in the NucleoSpin Trace Binding Strip/Binding Plate are created by addition of isopropanol to the lysate. The binding process is reversible and specific to nucleic acids. Contaminants are removed by two washing steps with ethanolic buffer. Pure genomic DNA is finally eluted under low ionic strength conditions in a slightly alkaline elution buffer.

Typically, yields of 1–2 µg genomic DNA can be purified from buccal swabs. The final concentration of eluted DNA ranges from 10–20 ng/µl (depending on the elution buffer volume) and the A260/280 is 1.8–1.9. The kits can be used manually under centrifugation or vacuum and can also be automated on common liquid handling instruments.

At-A-Glance Documents Images & Data Resources

Features

  • Time-saving parallel isolation of genomic DNA from forensic samples
  • Processing is possible under vacuum or by centrifugation
  • Suitable for manual and automated processing
  • Innovative MN Wash Plate minimizes risk of cross-contamination

Applications

Manual or automated isolation of genomic DNA from forensic samples:

  • Buccal swabs
  • Dried blood spots
  • Cigarette filters

Purified DNA is ready to use for any kind of enzymatic reactions (e.g., STR analysis).

Product Overview

NucleoSpin 8 Trace

NucleoSpin 96 Trace

Technology

Silica membrane

Silica membrane

Format

8-well strips

96-well plates

Processing

Manual or automated,
vacuum or centrifugation

Manual or automated,
vacuum or centrifugation

Starting Material

Forensic samples, buccal swabs, blood spots, cigarette filters

Forensic samples, buccal swabs, blood spots

Typical Yield

1–2 µg

1–2 µg

A260/280

1.8–1.9

1.8–1.9

Elution Volume

50–100 µl

50–100 µl

Preparation Time

30 min/6 strips

70 min/plate

Binding Capacity 

20 µg

20 µg


 
 
Products
Cat. # Product Package Size Price # of Units Select
740975 NucleoSpin® 8 Food 12 x 8 Preps $317.00
740975.5 NucleoSpin® 8 Food 60 x 8 Preps $1,335.00
740976.2 NucleoSpin® 96 Food 2 x 96 Preps $538.00
740976.4 NucleoSpin® 96 Food 4 x 96 Preps $998.00
740976.24 NucleoSpin® 96 Food 24 x 96 Preps $5,220.00
 

 

 

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