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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  Genomic_DNA >  Food >  Single_Prep

Isolation of Genomic DNA from Food & Feed—NucleoSpin Food

The NucleoSpin Food Kit allows you to quickly isolate genomic DNA from many types of processed food or animal feed of plant or animal origin. Food samples are highly heterogeneous and contain many different compounds such as fats and polysaccharides. These characteristics make DNA extraction more difficult. The NucleoSpin Food protocol guarantees good recovery rates for small genomic DNA fragments from food. We have optimized the lysis buffer and purification process to extract DNA from a variety of food products. The purified DNA is ready to use for all types of subsequent detection methods, especially as a template for PCR. Isolation of genomic DNA from food products assists in detecting and identifying contamination, genetically modified organisms (GMOs), or individual plant or animal species.

The NucleoSpin Food Kit allows you to process 50–200 mg of homogenized material using a microcentrifuge to speed the loading, washing, and elution steps. Due to the low DNA content of processed food, we recommend using 200 mg of starting material, which typically yields 0.1–10 µg DNA. The silica membrane spin technology has been optimized for high DNA recoveries and low binding of impurities, so the kit generates pure, ready-to-use DNA from any type of food.

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Features

  • Complete removal of PCR inhibitors
  • Purify low amounts of partially degraded DNA
  • Fast and easy procedure
  • Isolate DNA from various types of samples

Applications

  • Isolate DNA from complex matrices such as:
    • Processed food
    • Soy (milk and flour)
    • Chocolate
    • Cereals
    • Meat
    • Animal feed
  • Obtained DNA is suitable for:
    • Real-time PCR
    • Southern blotting
    • Enzymatic reactions

Product Overview

Technology Silica membrane
Format Mini spin columns
Starting Material 5–200 mg
Fragment Size 300 bp– >30 kb
Typical Yield 0.1–10 µg
A260/280 1.6–1.9
Elution Volume 100 µl
Preparation Time 30 min/6 preps
Binding Capacity 30 µg

 
 
Products
Cat. # Product Contents Size Price Units Select
740945.250 null 250 Preps $695.00
740945.50 null 50 Preps $157.00
 

 

 
 

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