Isolation of Genomic DNA from Food & Feed—NucleoSpin Food
The NucleoSpin Food Kit allows you to quickly isolate genomic DNA from many types of processed food or animal feed of plant or animal origin. Food samples are highly heterogeneous and contain many different compounds such as fats and polysaccharides. These characteristics make DNA extraction more difficult. The NucleoSpin Food protocol guarantees good recovery rates for small genomic DNA fragments from food. We have optimized the lysis buffer and purification process to extract DNA from a variety of food products. The purified DNA is ready to use for all types of subsequent detection methods, especially as a template for PCR. Isolation of genomic DNA from food products assists in detecting and identifying contamination, genetically modified organisms (GMOs), or individual plant or animal species.
The NucleoSpin Food Kit allows you to process 50–200 mg of homogenized material using a microcentrifuge to speed the loading, washing, and elution steps. Due to the low DNA content of processed food, we recommend using 200 mg of starting material, which typically yields 0.1–10 µg DNA. The silica membrane spin technology has been optimized for high DNA recoveries and low binding of impurities, so the kit generates pure, ready-to-use DNA from any type of food.