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Products >  Nucleic_Acid_Purification >  Genomic_DNA >  Blood >  Single_Prep

Isolation of Genomic DNA from Blood—Single Preps—NucleoSpin Blood, Blood QuickPure, Blood L/XL & NucleoBond CB

NucleoSpin Blood, Blood L, Blood XL, and Blood QuickPure kits are designed for isolating genomic DNA from whole blood, serum, plasma, buffy coats, and cultured cells in a convenient silica membrane-based spin column format. Maximum starting volumes range from 200 µl of blood for the NucleoSpin Blood and Blood QuickPure mini kits to 2 ml for the Blood L midi kit and 10 ml for the Blood XL maxi kit. The NucleoSpin Blood QuickPure kits are ultra-fast mini kits that include spin columns with a specially treated silica membrane which speeds up the procedure by allowing a combined washing/drying step. This reduces the number of washing and drying steps from three to only one, resulting in a hands-on time of less than 10 min. The DNA purified using any of these kits can be used directly for PCR, Southern blotting, or any kind of enzymatic reaction.

NucleoSpin Blood mini kits only (not the Blood QuickPure & Blood L/XL kits) may also be used for purifying viral DNA for a wide variety of viruses from blood and biological fluids (including HBV, CMV, HPV, TTV, and EBV). The proteinase K step included in the NucleoSpin Blood method removes viral proteins, allowing the viral DNA in the lysate to bind to the silica membrane. Since viral DNA copurifies with cellular DNA, we recommend using cell-free samples (serum or plasma) to prepare pure viral DNA. The purified DNA is suitable for typical downstream applications such as real-time PCR and enzymatic reactions.

NucleoBond CB

NucleoBond CB kits are designed for the purification of genomic DNA from blood, serum, or cell cultures using NucleoBond AXG anion exchange columns, which yield ultra-pure high molecular weight genomic DNA. Because NucleoBond columns run by gravity flow, shearing is kept to a minimum so that high molecular weight genomic DNA can be easily isolated. The DNA purified by this method is suitable for all kinds of subsequent reactions.

At-A-Glance Documents Images & Data Resources

Features

NucleoSpin Blood

  • DNA is ready to use in less than 30 min
  • Complete removal of PCR inhibitors
  • Suitable for whole blood treated with citrate, EDTA, heparin, CPDA
  • Also suitable for viral DNA isolation
  • Consistently high DNA yields
  • Cat. No. 740951.240C is designed for use with the QIAcube only

NucleoSpin Blood QuickPure

  • Ultra-fast procedure (10 min hands-on time)
  • Washing and drying step are combined into one step
  • Consistently high DNA yields
  • Elute in only 30–50 µl
  • Highly concentrated DNA, ready-to-use for sensitive downstream applications

NucleoSpin Blood L/XL

  • Medium- and large-scale DNA purification from blood
  • Complete removal of PCR inhibitors
  • Suitable for whole blood treated with citrate, EDTA, heparin, CPDA
  • Consistently high DNA yields

NucleoBond CB

  • Anion exchange-based kits for purification of up to 500 µg genomic DNA from whole blood
  • Ultra-pure genomic DNA from different samples
  • Kits for 100–500 µg genomic DNA
  • Starting sample size—up to 20 ml whole blood

Applications

NucleoSpin Blood

  • Genomic, bacterial, viral DNA isolation
  • DNA from whole blood (human, animal, fresh or frozen)
  • DNA from whole blood treated with citrate, EDTA, heparin, CPDA
  • DNA from serum, plasma, buffy coats, platelets, body fluids (e.g., amniotic fluid)
  • DNA from cultured cells
  • DNA suitable for:
    • PCR
    • Southern blotting
    • Enzymatic reactions

NucleoSpin Blood QuickPure & Blood L/XL

  • DNA from whole blood (human, animal, fresh or frozen)
  • DNA from whole blood treated with citrate, EDTA, heparin, CPDA
  • DNA from serum, plasma, buffy coats, platelets, body fluids (e.g., amniotic fluid)
  • DNA from cultured cells
  • DNA suitable for:
    • PCR
    • Southern blotting
    • Enzymatic reactions

NucleoBond CB

  • Genomic DNA from whole blood serum, plasma, buffy coat

Product Overview – NucleoSpin Blood & Blood QuickPure

NucleoSpin Blood

NucleoSpin Blood QuickPure

Technology

Silica membrane

Format

Mini spin columns

Starting Material

1–200 µl whole blood, serum, plasma, body fluids
<5 x 106 cultured cells

1–200 µl whole blood (human or animal, fresh or frozen), body fluids
<5 x 106 cultured cells (lymphocytes)
Fragment Size

200 bp–>30 kb

Typical Yield

4–6 µg

A260/280

1.6–1.9

Typical Concentration

40–100 ng/µl

80–120 ng/µl

Elution Volume

60–200 µl

30–50 µl

Preparation Time

~30 min/prep

~25 min/prep (<10 min hands-on-time)

Binding Capacity

60 µg

50 µg

Product Overview – NucleoSpin Blood L/XL

NucleoSpin Blood L

NucleoSpin Blood XL

Technology

Silica membrane

Format

Midi spin columns

Maxi spin columns

Starting Material

0.2–2 ml whole blood
5 x 106 cultured cells

2–10 ml whole blood
1 x 108 cultured cells

Fragment Size

200 bp–>30 kb

Typical Yield

40–60 µg

200–300 µg

A260/280

1.6–1.9

Typical Concentration

200–500 ng/µl

Elution Volume

120–200 µl

600–1,000 µl

Preparation Time

~60 min/prep

~60 min/prep

Binding Capacity

250 µg

700 µg

Product Overview – NucleoBond CB

NucleoBond CB 100

NucleoBond CB 500

Technology

Anion exchange chromatography

Format

Midi gravity-flow columns

Maxi gravity-flow columns

Starting Material

2–5 ml whole blood
<250 µl buffy coat
2 x 107 cultured cells

5–20 ml whole blood
<1 ml buffy coat
108 cultured cells

Fragment Size

500 bp–>300 kb

Typical Yield

100 µg

500 µg

A260/280

1.80–1.95

Preparation Time

4–5 hr

4–5 hr

Binding Capacity

100 µg

500 µg


 
 
Products
Cat. # Product Package Size Price # of Units Select
740508 NucleoBond® CB 100 20 Preps $231.00
740509 NucleoBond® CB 500 10 Preps $258.00
740951.250 NucleoSpin® Blood 250 Preps $487.00
740951.50 NucleoSpin® Blood 50 Preps $114.00
740951.240C NucleoSpin® Blood 240 Preps $487.00
740954.20 NucleoSpin® Blood L 20 Preps $142.00
740569.50 NucleoSpin® Blood QuickPure 50 Preps $115.00
740569.250 NucleoSpin® Blood QuickPure 250 Preps $498.00
740950.50 NucleoSpin® Blood XL 50 Preps $644.00
740950.10 NucleoSpin® Blood XL 10 Preps $132.00
 

 

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Cat. # Product Package Size Price # of Units Select
740562 NucleoBond® Small Rack Each $51.00
740563 NucleoBond® Large Rack Each $74.00
 
 

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