Features- Single-tube procedure minimizes risk of cross-contamination
- Small elution volumes: ≥50 µl for NucleoMag Blood 200 µl, ≥1 ml for NucleoMag Blood 3 ml
- Complete processing is possible at room temperature
- Easily adapted to automated use
- Consistently high yields
Applications- Manual or automated isolation of genomic DNA from whole blood
Obtain DNA that is ready for: - PCR
- Real-time PCR
- Southern blotting
- Enzymatic reactions
Product Overview| Technology | Magnetic bead technology | | Format | Highly reactive superparamagnetic beads | | Processing | 200-µl kit: manual or automated; 3-ml kit: automated*
| | Starting Material | 200 µl or 3 ml of whole blood (fresh or frozen, EDTA or citrate treated)
| | Fragment Size | 300 bp–approx. 50 kb | | Typical Yield | 2–8 µg (200 µl of blood) or 100–130 µg (3 ml of blood)
| | A260/280 | 1.6–1.9 | | Elution Volume | >50 µl (200-µl kit) or 1 ml (3-ml kit)
| | Preparation Time | 45 min/96 preps* (200-µl kit) or 60 min/24 preps* (3-ml kit)
| | Binding Capacity | Approx. 0.4 µg/µl |
*Established on KingFisher Flex.
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