Isolation of Genomic DNA from Whole Blood—NucleoMag Blood
NucleoMag Blood kits are designed for rapid, parallel purification of genomic DNA from whole blood in 96- or 24-well formats. After lysis of blood cells, genomic DNA is selectively bound to the NucleoMag B-Beads. Contaminants are washed away and the DNA can be eluted from the beads under low-salt conditions. Since the magnetic bead technology provides a closed system (no transfer of sample from one well to another is necessary during the preparation), the risk of cross-contamination is reduced. NucleoMag technology can be easily adapted to common liquid-handling instruments: It can be used either with static-pin separators (e.g., NucleoMag SEP) or magnetic separators integrated into robotic workstations. Purified DNA obtained with these kits is ready for use in PCR, Southern blotting, or any kind of enzymatic reaction.