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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  Genomic_DNA >  Blood >  Magnetic

Isolation of Genomic DNA from Whole Blood—NucleoMag Blood

NucleoMag Blood kits are designed for rapid, parallel purification of genomic DNA from whole blood in 96- or 24-well formats. After lysis of blood cells, genomic DNA is selectively bound to the NucleoMag B-Beads. Contaminants are washed away and the DNA can be eluted from the beads under low-salt conditions. Since the magnetic bead technology provides a closed system (no transfer of sample from one well to another is necessary during the preparation), the risk of cross-contamination is reduced. NucleoMag technology can be easily adapted to common liquid-handling instruments: It can be used either with static-pin separators (e.g., NucleoMag SEP) or magnetic separators integrated into robotic workstations. Purified DNA obtained with these kits is ready for use in PCR, Southern blotting, or any kind of enzymatic reaction.

  At-A-Glance   Documents   Images & Data

Features

  • Single-tube procedure minimizes risk of cross-contamination
  • Small elution volumes: ≥50 µl for NucleoMag Blood 200 µl, ≥1 ml for NucleoMag Blood 3 ml
  • Complete processing is possible at room temperature
  • Easily adapted to automated use
  • Consistently high yields

Applications

  • Manual or automated isolation of genomic DNA from whole blood

Obtain DNA that is ready for:

  • PCR
  • Real-time PCR
  • Southern blotting
  • Enzymatic reactions

Product Overview

Technology Magnetic bead technology
Format Highly reactive superparamagnetic beads
Processing 200-µl kit: manual or automated; 3-ml kit: automated*
Starting Material 200 µl or 3 ml of whole blood (fresh or frozen, EDTA or citrate treated)
Fragment Size 300 bp–approx. 50 kb
Typical Yield 2–8 µg (200 µl of blood) or 100–130 µg (3 ml of blood)
A260/280 1.6–1.9
Elution Volume >50 µl (200-µl kit) or 1 ml (3-ml kit)
Preparation Time 45 min/96 preps* (200-µl kit) or 60 min/24 preps* (3-ml kit)
Binding Capacity Approx. 0.4 µg/µl

*Established on KingFisher Flex.


 
 
Products
New Cat. # Product Contents Size Price Units Select
  744501.1 NucleoMag® Blood 200 µL 1 x 96 Preps $273.00
  744501.4 NucleoMag® Blood 200 µL 4 x 96 Preps $924.00
744502.1 NucleoMag® Blood 3mL 96 Preps $1,710.00
 

 

 
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