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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  Genomic_DNA >  Blood >  Magnetic

Isolation of Genomic DNA from Whole Blood—NucleoMag Blood

NucleoMag Blood 200 µl is designed for the rapid parallel purification of genomic DNA from whole blood in a 96-well format. After lysis of blood cells, genomic DNA is selectively bound to the NucleoMag B-Beads. Contaminants are washed away and the DNA can be eluted from the beads under low-salt conditions. Since the magnetic bead technology provides a closed system (no transfer of sample from one well to another is necessary during the preparation), the risk of cross-contamination is reduced. NucleoMag technology can be easily adapted to common liquid handling instruments: It can be used either with static-pin separators (e.g., NucleoMag SEP) or magnetic separators integrated into robotic workstations.

Typically, 96 samples can be purified in 45 minutes on common automation platforms. The kit provides reagents for the purification of 2–8 µg genomic DNA from 200 µl samples of whole blood. The purified DNA is ready to use for PCR, Southern blotting, and any enzymatic reactions.

At-A-Glance Documents Images & Data

Features

  • Single-tube procedure minimizes risk of cross-contamination
  • Small elution volume: ≥ 50 µl
  • Complete processing is possible at room temperature
  • Easily adapted to automated use
  • Consistently high yields

Applications

  • Manual or automated isolation of genomic DNA from whole blood

Obtain DNA that is ready for:

  • PCR
  • Real-time PCR
  • Southern blotting
  • Enzymatic reactions

Product Overview

Technology Magnetic bead technology
Format Highly reactive superparamagnetic beads
Processing Manual or automated
Starting Material 200 µl whole blood (fresh or frozen, EDTA or citrate treated)
Fragment Size 300 bp–approx. 50 kb
Typical Yield 2–8 µg
A260/280 1.6–1.9
Elution Volume >50 µl
Preparation Time 45 min/96 preps*
Binding Capacity Approx. 0.4 µg/µl

*Established on KingFisher Flex.


 
 
Products
Cat. # Product Package Size Price # of Units Select
744501.1 NucleoMag® Blood 200 µL 1 x 96 Preps $252.00
744501.4 NucleoMag® Blood 200 µL 4 x 96 Preps $850.00
 

 

 


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