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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  Genomic_DNA >  Blood >  HTP

Genomic DNA Isolation from Blood—NucleoSpin Blood 8/96 QuickPure, NucleoSpin 8/96 Blood & NucleoSpin 96 Blood Core Kit

The NucleoSpin 8/96 Blood QuickPure kits are designed for the rapid parallel isolation of genomic DNA from whole blood, serum, plasma, and body fluids (amniotic fluid, liquor, etc.)—in flexible 8-well strip or high-throughput 96-well plate formats which have been optimized for manual use. Animal or human blood samples and fresh or frozen material are processed conveniently via centrifugation of NucleoSpin Blood QuickPure Binding Strips/Plates.

The NucleoSpin Blood QuickPure method features a specially treated silica membrane which speeds up the procedure by allowing a combined washing/drying step. This reduces the number of washing and drying steps from 3 to only 1, minimizing hands-on time. Lysis is achieved by incubation of whole blood in a solution containing large amounts of chaotropic ions in the presence of proteinase K. Appropriate conditions for DNA binding to the silica membrane of the NucleoSpin Blood QuickPure Binding Strips/Plates are created by addition of ethanol to the lysate. Pure genomic DNA is eluted under low ionic strength conditions in a slightly alkaline elution buffer, and can be used directly for PCR, Southern blotting, or any kind of enzymatic reaction.

The NucleoSpin 8/96 Blood kits are designed for the rapid parallel isolation of genomic DNA from whole blood or cultured cells. After lysis of blood cells, genomic DNA is selectively bound to a silica membrane, while contaminants are thoroughly washed away. Elution is possible even under vacuum without cross-contamination. Due to its plate design and the possibility of performing the complete procedure under vacuum, NucleoSpin 8/96 Blood can be easily adapted to common liquid handling instruments for fully automated processing without manual interactions.

The NucleoSpin 96 Blood Core kit provides the buffers, Proteinase K, and NucleoSpin Blood Binding Plates only. Accessory plates (e.g., lysis plates, elution plates) are not provided with the core kit. The user can select additional consumables from a variety of suitable accessory plates according to individual needs, for the highest flexibility.

At-A-Glance Documents Images & Data Resources

Features

NucleoSpin 8/96 Blood QuickPure

  • Time-saving isolation of genomic DNA from whole blood
  • Optimized for manual use in a centrifuge
  • Reduced number of washing and drying steps minimizes hands-on time
  • Consistently high DNA yields

NucleoSpin 8/96 Blood

  • Optimized for manual or automated use under vacuum
  • Improved flow-rate reduces blockage of wells (no cross-contamination, reliable results)
  • Innovative MN Wash Plate minimizes risk of cross-contamination
  • Complete processing possible at room temperature
  • Consistently high yields

Applications

NucleoSpin 8/96 Blood QuickPure can be used for manual isolation of genomic DNA from:

  • Whole blood
  • Serum
  • Plasma
  • Buffy coats
  • Platelets
  • Body fluids
  • Cultured cells

NucleoSpin 8/96 Blood QuickPure can be used for automated isolation of genomic DNA from:

  • Whole blood
  • Cultured cells

Obtained DNA is ready to use for:

  • PCR
  • Southern blotting
  • Enzymatic reactions

Product Overview—NucleoSpin 8/96 Blood QuickPure

NucleoSpin 8 Blood QuickPure

NucleoSpin 96 Blood QuickPure

Technology

Silica membrane

Format

8-well strips

96-well plates

Processing

Manual, centrifugation

Starting Material

Human or animal whole blood, treated with EDTA, citrate, heparin, CPDA
200 µl whole blood
300 µl whole blood (use increased volumes of Lysis Buffer BQ1)
200 µl serum, plasma, buffy coats, body fluids
107 lymphocytes

Fragment Size

300 bp– >30 kb

Typical Yield

4–6 µg

A260/280

1.6–1.9

Elution Volume

75–100 µl

Preparation Time

60 min/12 strips

60 min/2 plates

Binding Capacity

60 µg

Product Overview—NucleoSpin 8/96 Blood

NucleoSpin 8 Blood

NucleoSpin 96 Blood
NucleoSpin 96 Blood Core Kit

Technology

Silica membrane

Format

8-well strips

96-well plates

Processing

Manual or automated, optimized for vacuum processing

Starting Material

Whole blood treated with EDTA, citrate, heparin, CPDA, human or animal blood
1–200 µl whole blood
2 x 106 cultured cells

Fragment Size

300 bp– >30 kb

Typical Yield

4–6 µg

A260/280

1.8–1.9

Elution Volume

100 µl

Preparation Time

35 min/6 strips

70 min/plate

Binding Capacity

20 µg


 
 
Products
Cat. # Product Package Size Price # of Units Select
740664 NucleoSpin® 8 Blood 12 x 8 Preps $271.00
740664.5 NucleoSpin® 8 Blood 60 x 8 Preps $1,131.00
740666 NucleoSpin® 8 Blood QuickPure 12 x 8 Preps $259.00
740666.5 NucleoSpin® 8 Blood QuickPure 60 x 8 Preps $1,101.00
740665.1 NucleoSpin® 96 Blood 1 x 96 Preps $355.00
740665.4 NucleoSpin® 96 Blood 4 x 96 Preps $917.00
740665.24 NucleoSpin® 96 Blood 24 x 96 Preps $5,131.00
740456.4 NucleoSpin® 96 Blood Core Kit 4 x 96 Preps $679.00
740667.2 NucleoSpin® 96 Blood QuickPure 2 x 96 Preps $541.00
740667.4 NucleoSpin® 96 Blood QuickPure 4 x 96 Preps $917.00
740667.24 NucleoSpin® 96 Blood QuickPure 24 x 96 Preps $5,131.00
 

 

 


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