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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  DNA_Clean-Up >  PCR_Clean-Up >  Spin

PCR Clean-Up and Gel Extraction Kits

With the NucleoSpin Gel and PCR Clean-Up Kits you get two applications in one. The supplied column, which is suitable for gel extraction as well as PCR purification, binds DNA to a silica membrane in the presence of a chaotropic salt. Once the binding mixture is loaded directly onto the NucleoSpin Gel and PCR Clean-Up Kit column, only a single wash step using Buffer NT3, an optimized buffer, is necessary—providing rapid DNA purification. You can isolate fragments from 50 bp–20 kb in a minimized elution volume (as low as 15 µl with the mini format, or 200 µl with the midi format) with a recovery of 70–95%. Downstream applications include sequencing, ligation, labeling, cloning, and restriction enzyme digestion.

The NucleoSpin Gel and PCR Clean-Up Kit was formerly known as the NucleoSpin Extract II Kit.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • ONE kit for PCR cleanup and gel extraction
  • Highly flexible—kit can be used for:
    • Small to large fragments (50 bp to 20 kb)
    • Low and standard elution volumes—elute in as little as 15 µl (mini) or 200 µl (midi)
    • Restriction digest cleanup
    • Samples containing detergent (kit has no detergent sensitivity)
  • Fast—no isopropanol precipitation step for PCR purification
  • Effective removal of primers

Applications

Purified DNA is suitable for:

  • Sequencing
  • Ligation
  • Labeling
  • Cloning
  • Restriction enzyme digestion

Product Overview

Technology Silica membrane
Format Mini or midi spin columns
Starting Material Mini: <400 µl PCR reaction mixture or <400 mg TAE/TBE agarose gel
Midi: <4 ml PCR reaction mixture or <4 g TAE/TBE agarose gel
Fragment Size 50 bp–20 kb
Typical Recovery 70–95%
A260/280 1.8–1.9
Elution Volume 15–30 µl (mini) or 200–400 µl (midi)
Preparation Time 10 min/6 preps (mini) or 25 min/6 preps (midi)
Binding Capacity 25 µg (mini) or 75 µg (midi)

 
 
Products
New Cat. # Product Contents Size Price Units Select
740986.20 NucleoSpin® Gel & PCR Clean-up Midi 20 Preps $202.00
  740609.250 NucleoSpin® Gel and PCR Clean-Up 250 Preps $389.00
  740609.50 NucleoSpin® Gel and PCR Clean-Up 50 Preps $85.00
  740609.240C NucleoSpin® Gel and PCR Clean-Up 240 Preps $389.00
 

 

Recommended Products
Cat. # Product Size Price Units Select
740595.150 Buffer NTB 150 mL $30.00
740654.100 Buffer NTC 100 mL $36.00
 
 
 

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